General laboratory chemicals and reagent solutions were purchased from Sigma-Aldrich (St. Louis, MO). ELISA kits for IL-6 and TNF-α were purchased from Bio-legend. ELISA kit for CXCL1 was purchased from R&D systems. All antibodies were purchased from Santacruz unless otherwise specified. LPS was purchased from Sigma Aldrich. Colitis grade DSS (36,000–50,000 M.W) was purchased from MP Bio. UroA was custom synthesized as previously described23 (link).
General laboratory chemicals
Manufactured by Merck Group
Sourced in Germany, United States
General laboratory chemicals are a diverse range of substances used in various scientific and research applications. They serve as essential tools for conducting experiments, analyses, and other laboratory procedures. These chemicals enable researchers and scientists to carry out necessary processes, reactions, and measurements in a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Colitis grade dss, by MP Biomedicals (1 mentions)
Elisa kits for il 6, by BioLegend (1 mentions)
Elisa kit for cxcl1, by R&D Systems (1 mentions)
Tnf α, by BioLegend (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Anti gli1, by Cell Signaling Technology (1 mentions)
Anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti shh, by Cell Signaling Technology (1 mentions)
Anti ser9 phospho gsk 3β, by Cell Signaling Technology (1 mentions)
Anti numb, by Cell Signaling Technology (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Lipopolysaccharides (lps), by Immunotools (1 mentions)
Oxyresveratrol, by Merck Group (1 mentions)
Sodium phosphate monobasic dihydrate, by Merck Group (1 mentions)
Hplc ms grade acetonitrile, by Merck Group (1 mentions)
Milli q integral 15 system, by Merck Group (1 mentions)
Mulberroside a, by Merck Group (1 mentions)
3 protocols using general laboratory chemicals
1
Inflammatory Bowel Disease Assays
2
Molecular Regulatory Mechanisms in Cell Signaling
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General laboratory chemicals were obtained from Sigma-Aldrich or Merck Millipore (Darmstadt, Germany). TNF-α and TRAIL were purchased from PeproTech Inc (Rocky Hill, NJ, USA). Pam3CSK4, LPS and R848 were purchased from ImmunoTools (Friesoythe, Germany). Anti-β-ACTIN antibody was bought from Sigma-Aldrich. Anti-p53 and anti-COP1 antibodies were obtained from Santa Cruz Biotechnology Ltd (Dallas, Texas, USA). Anti-SHH, anti-GLI1, anti-PTCH1, anti-NUMB, anti-Ser9 phospho GSK-3β and anti-UbK48 were purchased from Cell Signaling Technology (Danvers, MA, USA). Annexin V- fluorescein isothiocyanate (FITC) was from Miltenyi Biotech (Bergisch Gladbach, Germany). HRP conjugated anti-rabbit IgG and anti-mouse IgG were obtained from Jackson ImmunoResearch (West Grove, PA, USA).
3
Bioactive Compounds Extraction from Morus alba Twigs
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Mulberroside A, p‐coumaric acid, oxyresveratrol, (+)‐catechin hydrate, formic acid, AAPH and FL sodium salt with high purity were purchased from Sigma‐Aldrich, and HPLC‐grade water, HPLC‐MS‐grade acetonitrile and methanol were purchased from Merck. General laboratory chemicals were obtained from Sigma‐Aldrich Chemical Co. (St Louis, MO, USA) and Merck Millipore (Darmstadt, Germany) unless otherwise specified. HPLC‐grade acetonitrile and methanol, sodium phosphate dibasic dihydrate and sodium phosphate monobasic dihydrate were obtained from Sigma‐Aldrich. ACS reagent‐grade ammonium acetate was purchased from VWR (Radnor, PS, USA). HPLC‐grade water was prepared using a Milli‐Q Integral 15 system (Merck Millipore). Morus alba twigs were collected in 2018 from the city of Chongqing, western China and authenticated by an external consultant. A voucher specimen has been deposited at the Herbarium facility at L'Oreal (Advanced Research, Bangalore), India, under the voucher specimen number MA/L'Oreal/R&D‐0014.
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