Slitpz05248

The following strains were obtained from the Bloomington Drosophila Stock Center: OregonR, w¹¹¹⁸, lea², lea^EP2582, UAS-lea^RNAi, UAS-Slit, pros³⁸ (pros-GFP), slit^PZ05248, esg^k00606, P{GawB}386Y, UAS-Flp [#5254] and act>y>Gal4,UAS-GFP [#4411], UAS-mCherry. UASSlit^RNAi(TRiP1) and UAS-Slit^RNAi(TRiP2) are from the Transgenic RNAi Project, stocks JF01228 and JF01229. UAS-Slit^RNAi(VDRC) and UAS-Prospero^RNAi were obtained from the Vienna Drosophila RNAi Center (transformant ID 20210 and 101477 respectively). The line esgGal4^NP5130 was kindly provided by S. Hayashi, DeltaGal4 and Su(H)GBEGal4 by S. Hou, UAS-Notch^RNAi by N. Perrimon UAS-Notch^intra by M. Rand, actin5cGal4Geneswitch(255) by J. Towers and NP1Gal4 by D.Ferrandon.
The UAS-lea^RNAi, UAS-Slit^RNAi(VDRC) and UAS-Pros^RNAi were validated and successfully used in previous studies (Brierley et al., 2009 (link); Neumuller et al., 2011 (link); Tayler et al., 2004 (link)).
Flies were raised on standard yeast and molasses - based food, at 25°C and 65% humidity, on a 12 h light/dark cycle, unless otherwise indicated.

The following fly strains were used: esg-Gal4 line (from Shigeo Hayashi); Su(H)GBE-Gal4 (Zeng et al., 2010 (link)), Su(H)GBE-tub-Gal80, and ISC^ts (esg-Gal4, UAS-GFP; tub-Gal80^ts, Su(H)GBE-tub-Gal80) (Zeng and Hou, in Press), and UAS-CycT were generated in our laboratory. The following strains were obtained from the Bloomington Drosophila Stock Center (BDSC): tub-Gal80^ts, lea², lea^EP2582, UAS-p35, N^264-39, UAS-lea^RNAi (BL9286), slit^PZ05248, UAS-slit^RNAi-1 (BL31468), UAS-robo3^RNAi-1 (BL29398), UAS-CycT^RNAi-1 (BL31762), UAS-CycT^RNAi-2 (BL32976), UAS-Cdk9^RNAi (BL34982), CycT^j11B2 (BL12101), and fly lines used for MARCM clones, including FRT^2A-piM, FRT^40A-piM, SM6,hs-flp, MKRS,hs-flp, FRT^2Atub-Gal80, FRT^40Atub-Gal80, and FRT^19Atub-Gal80. The following transgenic RNAi lines were obtained from VDRC: UAS-slit^RNAi-2 (v108853), UAS-robo1^RNAi (v42579), and UAS-robo3^RNAi-2 (v44702). CycT^RS5 (Kyoto125610) was from the Kyoto Drosophila Stock Center. Flies were raised on standard fly food at 25°C and 65% humidity, unless otherwise indicated.