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Dynabeads cd8

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Dynabeads CD8 are magnetic beads coated with antibodies specific to the CD8 surface antigen. They are used for the isolation and enrichment of CD8-positive cells from biological samples.

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Lab products found in correlation

Human cxcl13 quantikine elisa kit, by R&D Systems (2 mentions) Human high sensitivity cytokine premixed kit b, by R&D Systems (2 mentions) Bio plex 200 array system, by Bio-Rad (2 mentions)

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Dynabeads (3 citations)

2 protocols using dynabeads cd8

1

Cytokine Profiling of HIV-specific CD8+ T cells

Cited 1 time
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One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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2

Cytokine Profiling of HIV-specific CD8+ T cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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