A small sample of thymus, approximately 1 mm3, was fixed in 2.5% glutaraldehyde and 2.5% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) overnight and then in 1% osmium tetroxide at 4 °C, postfixed, dehydrated, clarified, infiltrated in epoxy resin, and polymerized at 60 °C for 24 h. Samples were then prepared to obtain semi-thin sections (1 μm) stained with toluidine blue and ultrathin sections (60 nm) stained with uranyl acetate for 10–15 min and lead citrate for 7 min, which were then examined using a JEOL transmission electron microscope (100 CX; Tokyo, Japan) at the Faculty of Science, Alexandria University, Alexandria, Egypt.
Transmission electron microscope 100 cx
Manufactured by JEOL
Sourced in Japan
The JEOL Transmission Electron Microscope (100 CX) is a high-performance electron microscope designed for advanced research and analysis. It utilizes a beam of electrons to produce magnified images of microscopic samples, providing detailed information about their structure and composition at the nanoscale level.
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Lab products found in correlation
2 protocols using transmission electron microscope 100 cx
1
Ultrastructural Analysis of Thymus Tissue
2
Ultrastructural Analysis of Lung Tissue
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Small fragments of right lungs were fixed in 2.5% buffered glutaraldehyde then post fixed with osmium tetroxide, dehydrated, cleared and then infiltrated in epoxy resin [28 ]. Semi-thin sections (cut at 1 μm) were obtained and stained with toluidine blue. Ultrathin sections were sectioned and stained with uranyl acetate and lead citrate then examined using a JEOL transmission electron microscope (100 CX; Tokyo, Japan) at the Faculty of Science, Alexandria University, Alexandria, Egypt.
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