Parbendazole

Manufactured by Merck Group

Sourced in United States

Parbendazole is a laboratory product manufactured by Merck Group. It is a white to off-white crystalline powder used in various research and analytical applications. The core function of Parbendazole is to serve as a reference standard or analytical tool in laboratory settings.

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Lab products found in correlation

4 protocols using parbendazole

Parbendazole Impact on Paramecium Learning

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Parbendazole was acquired from Sigma-Aldrich, USA. We incubated Paramecium in a culture solution with a 100 μM concentration of Parbendazole for 24 h prior to the experimental test for the Parbendazole group. This time interval was selected based on the
Pape et al. report (Pape et al., 1991) we screened a wide variety of such compounds for their effects on the growth of Paramecium tetraurelia cell cultures. Compounds tested include agents of widely different chemical composition and with reported effects on widely different cell types. We can differentiate between different drug effects: (a, indicating the presence of Parbendazole’s effect after 24 h. Parbendazole disrupts the microtubular dynamics of the Paramecium, so it should negatively affect its learning behavior if microtubules are involved in Paramecium’s learning.

Alipour A., Hatam G, & Seradj H. (2022). Microtubule Disruption Without Learning Impairment in the Unicellular Organism, Paramecium: Implications for Information Processing in Microtubules. Basic and Clinical Neuroscience, 13(1), 139-152.

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Topography-Mediated hMSC Adhesion Modulation

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hMSCs were seeded on Topo1018 surface topographies at the seeding density of 5,000 cells/cm² in a basic medium supplemented with manganese (II) chloride (MnCl₂) (Sigma-Aldrich) at a final concentration of 2 mM to activate integrins, paclitaxel (Sigma-Aldrich) at a final concentration of 1 µM to stabilize microtubule arresting, parbendazole (Bio-Connect B.V) at a final concentration of 4 µM to inhibit the microtubule assembly. As paclitaxel and parbendazole were dissolved in DMSO, we added a DMSO control, and its concentration in the final culture media was 4 µM. We also added RGD peptides Gly-Arg-Gly-Asp-Ser-Pro and Gly-Arg-Gly-Asp-Ser-Pro-Lys (Sigma-Aldrich) to block integrin activation, Gly-Arg-Ala-Asp-Ser-Pro (Sigma-Aldrich) as a negative control. Cells were exposed to pharmaceutical reagents for 4 h upon cell seeding.

Dede Eren A., Lucassen A.W., Tuvshindorj U., Truckenmüller R., Giselbrecht S., Eren E.D., Tas M.O., Sudarsanam P, & de Boer J. (2022). Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton. Frontiers in Cell and Developmental Biology, 10, 863721.

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BEAS-2B Cell Culture and Treatment

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BEAS-2B normal human epithelial cell line was purchased from ATCC (Catalog number: CRL-9609) and cultured according to vendor instructions using BEGM kit from LONZA (Catalog number: CC-3170). Cells were cultured on 96-well black μ-plate from ibidi (Catalog Number: 89626) for imaging studies. Tanespimycin (abcam ab141433), Acetylcysteine (Cayman, 20261), Amifostine (Cayman 14398), Bortezomib (Ayman 10008822), FK-866 (Cayman 13287), Gemcitabine (Cayman 11690), Idarubicin (Cayman 14176), NVP-AUY922 (Cayman 10012698), NVP-BEZ235 (Cayman 10565), PIK-75 (Cayman 10009210), SN-38 (Cayman 15362), Tretinoin (Cayman 11017), YM-155 (Cayman 11490), Ingenol (Cayman 14031), Sulforaphane (LKT S8044), CD-437 (Sigma C5865), and Parbendazole (Sigma 1498706) were dissolved in DMSO. 1000x concentration working solution was used for downstream experimentation.

Asano T., Chelvanambi S., Decano J.L., Whelan M.C., Aikawa E, & Aikawa M. (2022). In silico Drug Screening Approach Using L1000-Based Connectivity Map and Its Application to COVID-19. Frontiers in Cardiovascular Medicine, 9, 842641.

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Anticancer Effects of Benzimidazole Derivatives

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Pancreatic cancer (PC) cell lines AsPC-1 and Capan-2 were acquired from Cell Lines Service (CLS, Eppelheim, Germany). AsPC-1 carries mutations both in KRAS and p53, while Capan-2 is KRAS mutated and p53 wild type. Cells were cultured in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) at 37 °C, 5% CO₂. Parbendazole, fenbendazole, mebendazole and oxibendazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gemcitabine was obtained by Selleckchem (Houston, TX, USA). DMSO was used to prepare stock solutions of the different drugs. A fixed amount of DMSO was present in each experiment and the maximum percentage of the solvent in culture media (0.15%) was employed in drug combination experiments.

Florio R., Veschi S., di Giacomo V., Pagotto S., Carradori S., Verginelli F., Cirilli R., Casulli A., Grassadonia A., Tinari N., Cataldi A., Amoroso R., Cama A, & De Lellis L. (2019). The Benzimidazole-Based Anthelmintic Parbendazole: A Repurposed Drug Candidate That Synergizes with Gemcitabine in Pancreatic Cancer. Cancers, 11(12), 2042.

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