Participants were instructed to fast (except water) overnight before blood sampling. They were then requested to sit quietly and relax for 25–45 min prior to obtaining the blood samples. The sBDNF level was quantified using the Quantikine® ELISA Human BDNF Immunoassay (R&D Systems, Inc., Minneapolis, MN, USA) at the Global Clinical Central Lab (Yongin, Korea). The sBDNF level was classified into tertiles (high, middle, or low). Additionally, the patients were divided according to the median sBDNF level into higher and lower groups.
Quantikine elisa human bdnf immunoassay
Manufactured by R&D Systems
Sourced in United States
The Quantikine® ELISA Human BDNF Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human brain-derived neurotrophic factor (BDNF) levels in cell culture supernates, serum, and plasma.
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6 protocols using quantikine elisa human bdnf immunoassay
1
Serum BDNF Levels in Fasted Participants
2
Serum BDNF Quantification Protocol
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12-hour fasting blood samples were collected, processed, and stored at the Clinical Research and Clinical Trials Laboratory at Hamilton General Hospital. Samples were processed within two hours of collection. After 30 minutes of clotting time, samples were spun at 1500 × g (3000 rpm) for 15 minutes until blood was well separated. Samples were then aliquotted into 2 mL cryovials and stored in liquid nitrogen for future analyses. Serum BDNF level was assayed using Quantikine® ELISA Human BDNF Immunoassay (R&D Systems Inc.). All analyses were conducted blindly according to standard procedures.
3
Quantitative Analysis of Bcl-2 and BDNF in Cultured Cells
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The concentration of protein in the cultured cells was measured using an ELISA human immunoassay kit: Human Bcl-2 ELISA Kit (Elabscience, Wuhan, China, catalog no. E-EL-H0114) and Quantikine ELISA Human BDNF Immunoassay (R&D Systems, McKinley Place, MN, USA catalog no. DBD00). The absorbance was read at 450 nm using an ELX 800 IU automated Microplate Reader (Bio-Tek Instruments, Inc., Winooski, VT, USA; Gene 5 Software V 3.02). The results were analyzed using a quadratic log–log curve fit.
4
Serum BDNF Levels Measurement Protocol
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Samples were collected and handled as described in Samaan et al. [19 (link)]. Two-hour fasting blood samples were collected, allowed 30 min clotting time, and spun at 1500 g for 15 min ensuring adequate blood separation. Samples were aliquoted in cryovials within 2 h of collection and stored at −196 °C (liquid nitrogen) at the Clinical Research and Clinical Trial Laboratory in Hamilton, Ontario. Serum BDNF levels were measured in the samples with a Quantikine® ELISA Human BDNF Immunoassay (R&D Systems Inc., Minneapolis, MN, USA). Sample analysis was conducted in a blinded fashion to avoid bias.
This study is reported in accordance with the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines [20 (link)].
This study is reported in accordance with the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines [20 (link)].
5
Serum BDNF Biomarker Stratification
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Participants were instructed to fast from the previous night before morning blood sampling. They sat for 25-45 min quietly and relaxed before blood samples were acquired. Serum BDNF levels were determined using Quantikine ELISA Human BDNF Immunoassay (R&D Systems, Minneapolis, USA) at Global Clinical Central Lab (Yongin, Korea). Based on the median value of BDNF levels, participants were divided into higher and lower BDNF groups.
6
Serum BDNF Level Assessment
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Participants were instructed to fast (except water) overnight prior to blood sampling. Participants were then asked to sit quietly and relax for 25-45 min before obtaining the blood samples.
The sBDNF level was measured using the Quantikine® ELISA Human BDNF Immunoassay (R&D Systems, Inc., Minneapolis, MN, USA) at the Global Clinical Central Lab (Yongin, Korea). The sBDNF level was classi ed into tertiles (high, middle, or low). Additionally, the patients were divided according to the median sBDNF level into higher and lower groups.
The sBDNF level was measured using the Quantikine® ELISA Human BDNF Immunoassay (R&D Systems, Inc., Minneapolis, MN, USA) at the Global Clinical Central Lab (Yongin, Korea). The sBDNF level was classi ed into tertiles (high, middle, or low). Additionally, the patients were divided according to the median sBDNF level into higher and lower groups.
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