Rabbit anti p21 waf1 cip1

Chemicals were purchased from Sigma-Aldrich (Deisenhofen, Germany) except Mdivi-1 (Enzo Life Science, Lörrach, Germany) as well as doxycycline, blasticidin and zeocin (Invivogen, San Diego, CA, USA). Cell culture materials and media were obtained from Biochrom (Berlin, Germany). MitoTracker Green^FM (MTG) and MitoSOX™ Red superoxide indicator (MitoSOX) were purchased from Molecular Probes (Eugene, USA).
The following primary antibodies were used: rabbit anti-DNP (Sigma-Aldrich, Deisenhofen, Germany), rabbit anti-PINK1 (Cell Signaling, Boston, MA, USA), mouse anti-β-actin (Cell Signaling, Boston, USA), rabbit anti-COX IV (Cell Signaling, Boston, MA, USA), rabbit anti-Lon protease (Abcam, Cambridge, UK), mouse anti-GAPDH (Abcam, Cambridge, UK), rabbit anti-Ki-67 (Abcam, Cambridge, UK), mouse anti-CDKN2A/p16INK4α (Abcam, Cambridge, UK), rabbit anti-p21 Waf1/Cip1 (Cell Signaling, Boston, MA, USA), mouse anti-MT-CO1 (Abcam, Cambridge, UK), mouse anti-SDHA (Abcam, Cambridge, UK), mouse anti-VDAC (Abcam, Cambridge, UK) and rabbit anti-Fis1 (Cell Signaling, Boston, MA, USA). Secondary antibodies used for immunoblotting were purchased from LI-COR Biosciences (Lincoln, AL, USA). The FITC-labeled antibody for immunofluorescence was purchased from Invitrogen (Carlsbad, CA, USA).

Cells were lysed in RIPA lysis buffer (Cat# P0013K, Beyotime, China) added with Protease inhibitors cocktail. Equal volumes of proteins were separated by 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred onto a polyvinylidence difluoride (PVDF) membrane. Primary rabbit anti-FECH (Cloud-Clone, Wuhan, China), rabbit anti-Ferritin (Abcam, Cambridge, UK), rabbit anti-GPX4 (HuaBio, Hangzhou, China), rabbit anti-p21-Waf1/Cip1 (Cell Signaling Technology, Danvers, USA), rabbit anti-p16-INK4A (Proteintech, Chicago, USA), rabbit anti-LC3 (Proteintech, Chicago, USA), rabbit anti-p62 (Cell Signaling Technology, Danvers, USA), rabbit anti-actin (Cloud-Clone, Wuhan, China), and secondary anti-rabbit HRP-IgG antibodies (Cell Signaling Technology, Danvers, USA) were used for immunoblotting.