Sw837

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SW837 is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed for general laboratory use, but a detailed functional description is not available without the risk of making unsupported claims. As a Marketing Specialist, I aim to provide factual information without extrapolation or interpretation.

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Lab products found in correlation

10 protocols using sw837

Colorectal cancer cell line analysis

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Colorectal cancer cell lines DLD-1, RKO and SW837 were obtained from the American Type Culture Collection (Manassas, VA, USA). DLD-1 and SW837 cells were cultured in RPMI1640 medium and RKO cells were cultured in DMEM/F-12 medium, both supplemented with antibiotics and 10% foetal bovine serum (Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO₂. Near-diploid (2N) and near-tetraploid (4N) clones of DLD-1 and RKO cell lines were previously generated in our laboratory^{16 (link)}. Cytokinesis blockage with 1.5 μg/ml of dihydrocytochalasin B (Sigma-Aldrich, St. Louis, MO, USA) for 24 h was performed in double thymidine treated SW837 to establish 4N clones. Wild-type and post-tetraploid clones derived from the hTERT-immortalized retinal-pigmented epithelial cells (RPE1) (kindly provided by Z. Storchova, University of Kaiserslautern, Kaiserslautern, Germany) were cultured in DMEM/F-12 medium supplemented with antibiotics and 10% FBS at 37 °C in 5% CO₂. For the experiments described in this study, we used one 2N clone and two 4N clones derived from the DLD-1, RKO and SW837 cell lines. As for the RPE1 cells, we used one 2N and one 4N clone.

Galofré C., Asensio E., Ubach M., Torres I.M., Quintanilla I., Castells A, & Camps J. (2020). Centrosome reduction in newly-generated tetraploid cancer cells obtained by separase depletion. Scientific Reports, 10, 9152.

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Culturing Human Colorectal Cancer Cell Lines

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The human CRC cell lines such as SW620, Ls-174T, SW480, HCT15, LOVO, SW837, HT29, HCT116, and DLD1 were obtained from the American Type Culture Collection (ATCC, USA). SW620 and SW837 were cultured in DMEM (Invitrogen, USA) with 10% fetal bovine serum (FBS, Gibco), and Ls-174T, SW480, HCT15, LOVO, HT29, HCT116, and DLD1 were maintained in RPMI-1640 medium (Invitrogen, USA) with 10% FBS at 37 °C in a humidified atmosphere with 5% CO₂.

Wang Y., Xu H., Jiao H., Wang S., Xiao Z., Zhao Y., Bi J., Wei W., Liu S., Qiu J., Li T., Liang L., Ye Y., Liao W, & Ding Y. (2018). STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway. Cell Death & Disease, 9(6), 664.

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Cell Line Cultivation and Maintenance

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293T, NCCIT, U87, SW837, MCF-7 and HepG2 cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). U251 cells were obtained from Shanghai Bogoo Biotechnology, Co., Ltd. (Shanghai, China). HCCLM3 cells were purchased from the Cell Bank of the Chinese Academic of Sciences, (Shanghai, China). L3.6pl cells, derived from human pancreatic carcinoma (28 (link)), were a gift from Professor M.H. Wang (Cancer Biology Research Center, School of Pharmacy, Texas Technical University Health Sciences Center, Amarillo, TX, USA). Most cells were cultured in DMEM (21063-029, Invitrogen, Carlsbad, CA, USA), SW837 cells were maintained in RPMI-1640 medium (11835-030, Invitrogen) and L3.6pl cells were cultured in MEM (51200-038, Invitrogen), all supplemented with 10% heat-inactivated fetal bovine serum (FBS) (10099, Gibco, Carlsbad, CA, USA) and 1% (v/v) penicillin/streptomycin (PS) (15140-148, Gibco). The cells were cultured at 37°C in a humidified 5% CO₂ incubator (3111, Thermo Fisher Scientific, Waltham, MA, USA).

ZHAO Q.W., ZHOU Y.W., LI W.X., KANG B., ZHANG X.Q., YANG Y., CHENG J., YIN S.Y., TONG Y., HE J.Q., YAO H.P., ZHENG M, & WANG Y.J. (2015). Akt-mediated phosphorylation of Oct4 is associated with the proliferation of stem-like cancer cells. Oncology Reports, 33(4), 1621-1629.

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Colorectal Cancer Tissue Collection and Cell Lines

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A total of 100 cases of fresh CRC tissues and their matched adjacent normal tissues were collected after curative surgical operations from the Department of General Surgery, Nanfang Hospital (Guangzhou, China) from February 2014 to January 2015. All tissue biopsies were freshly frozen in liquid nitrogen until further use. None of the patients underwent chemotherapy, radiotherapy and immunotherapy and they had been diagnosed with colorectal columnar adenocarcinoma by Hematoxylin-Eosin (HE) staining. The clinic pathological information including age, gender, differentiation degree and TNM (T, invasive depth; N, lymph node metastasis and M, distant metastasis) stage of the patients were collected. Informed consent was obtained from all patients before surgery informed consent and approval by the ethics committee of Southern Medical University Institutional Board (Guangzhou, China).
The human CRC cell lines SW480, SW620, SW837, HCT116, HCT15, LOVO and normal colorectal mucosa cell FHC were obtained from American Type Culture Collection (ATCC). SW620 and SW837 were cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS, Gibco); FHC, SW480, HCT116, HCT15 and LOVO were maintained in RPMI-1640 medium (Invitrogen) with 10% FBS.

Wang Y.X., Chen Y.R., Liu S.S., Ye Y.P., Jiao H.L., Wang S.Y., Xiao Z.Y., Wei W.T., Qiu J.F., Liang L., Liao W.T, & Ding Y.Q. (2016). MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42. Oncotarget, 7(51), 84826-84838.

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Cultivation of Human CRC Cell Lines

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The human CRC cell lines SW480, RKO, HCT116 and SW837 were purchased from American Type Culture Collection. SW480, RKO, SW837 and HCT116 cells were cultured in RPMI 1640 medium (Gibco). All the medium was added with 10% FBS (Gibco). All the cells were cultured at 37 °C with 5% CO₂.

Wang S., Yan S., Zhu S., Zhao Y., Yan J., Xiao Z., Bi J., Qiu J., Zhang D., Hong Z., Zhang L., Huang C., Li T., Liang L., Liao W., Jiao H., Ding Y, & Ye Y. (2018). FOXF1 Induces Epithelial-Mesenchymal Transition in Colorectal Cancer Metastasis by Transcriptionally Activating SNAI1. Neoplasia (New York, N.Y.), 20(10), 996-1007.

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Colorectal Cancer Cell Line Culture

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Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and the 293 T cell line was obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy’s 5A (Gibco, Carlsbad, CA, USA), while SW480, SW620, SW48, SW837 and 293 T cells were cultured in D MEM (Gibco, Carlsbad, CA, USA). NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA). All culture media contained 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidified atmosphere of 5% CO₂ at 37 °C.

Xu H., Liu Y., Cheng P., Wang C., Liu Y., Zhou W., Xu Y, & Ji G. (2020). CircRNA_0000392 promotes colorectal cancer progression through the miR-193a-5p/PIK3R3/AKT axis. Journal of Experimental & Clinical Cancer Research : CR, 39, 283.

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Culturing Colorectal Cancer Cell Lines

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The human CRC cell lines HCT116, SW480, SW620, HT29, CaCo2, Colo205, DLD1, LS174T, HCT15, HCT8, RKO, SW837, and LoVo were purchased from the American Type Culture Collection and were stored in the Department of Pathology, Southern Medical University, China. SW620, SW480, and SW837 cells were cultured in Leibovitz’s L-15 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco). HCT116 cells were cultured in McCoy’s 5A medium (Gibco) with 10% FBS. LoVo, CaCo2, and HT29 cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS. HCT15, DLD1, LS174T, HCT8, RKO, and Colo205 cells were cultured in RPMI 1640 medium (Gibco) with 10% FBS. Human umbilical vein endothelial cells (HUVECs) were cultured in DMEM containing F12K medium (Gibco) and 20% FBS (Gibco). All cells were cultured at 37°C in a 5% CO₂ atmosphere.

Qiu J., Li M., Su C., Liang Y., Ou R., Chen X., Huang C., Zhang Y., Ye Y., Liao W, & Zhang C. (2022). FOXS1 Promotes Tumor Progression by Upregulating CXCL8 in Colorectal Cancer. Frontiers in Oncology, 12, 894043.

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Culturing Human Colorectal Cancer Cell Lines

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The human CRC cell lines SW480, HT29, HCT15, HCT116, SW620, LS174t, SW837, LOVO, DLD1 and RKO were purchased from the American Type Culture Collection. SW620, HT29 and LOVO cells were cultured in DMEM medium (Gibco) supplemented with 10% foetal bovine serum (FBS) (Gibco). SW480, HCT116, HCT15, LS174t, SW837 and DLD1 cells were cultured in RPMI 1640 medium (Gibco) with 10% FBS (Gibco).

Wang S., Qiu J., Liu L., Su C., Qi L., Huang C., Chen X., Zhang Y., Ye Y., Ding Y., Liang L, & Liao W. (2020). CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET. Journal of Experimental & Clinical Cancer Research : CR, 39, 168.

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Culturing Diverse Colorectal Cancer Cell Lines

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Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and the 293T cell line was obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy's 5A (Gibco, Carlsbad, CA, USA), while SW480, SW620, SW48, SW837 and 293T cells were cultured in D MEM (Gibco, Carlsbad, CA, USA). NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA).
All culture media contained 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidi ed atmosphere of 5% CO 2 at 37 °C.

Xu H., Liu Y., Cheng P., Wang C., Liu Y., Zhou W., Xu Y., & Ji G. (2020). CircRNA_0000392 promotes colorectal cancer progression through the miR-193a-5p/PIK3R3/AKT axis.

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Culture of Colorectal Cancer Cell Lines

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Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and 293 T cell lines were obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy's 5A (Gibco, Carlsbad, CA, USA), SW480, SW620, SW48, SW837 and 293 T cells were cultured in D MEM (Gibco, Carlsbad, CA, USA), NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA), all culture medium containing 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidified atmosphere of 5% CO2 at 37 °C.

Xu H., Liu Y., Cheng P., Wang C., Liu Y., Zhou W., Xu Y., & Ji G. (2020). CircRNA_0000392 Promotes Colorectal Cancer Progression by Sponging miR-193a-5p.

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