Og n octyl glucoside

1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (PS), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (PE), 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) (PG), and brain PI(4,5)P2 were purchased from Avanti polar lipids; DDM (n-dodecyl β-d-maltoside) and OG (n-octyl glucoside) were from Gold Biotechnology; IPTG was from Research Products International Corp.; Triton X-100 was from Sigma Aldrich; Ni-Sepharose 6 Fast Flow was from GE Healthcare; 2-mercaptoethanol and glycerol were from Thermo Fischer Sc.; Bio-beads SM2 were from BIO-RAD; ATP-γ-S was from Abcam.

1,2-Dioleoyl-sn-glycero-3-phospho-l-serine (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (PG), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (PE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE), and brain phosphatidylinositol 4,5-bisphosphate or PtdIns(4,5)P2 (PI[4,5]P2) were purchased from Avanti Polar Lipids; DDM (n-dodecyl β-D-maltoside) and OG (n-octyl glucoside) were from Gold Biotechnology; IPTG (isopropyl β-d-1-thiogalactopyranoside), Triton X-100, Hepes, KCl, imidazole, MβCD (methyl-β-cyclodextrin), and DSP (dithiobis succinimidyl propionate) were from Sigma-Aldrich; glutathione Sepharose 4 B and Ni Sepharose 6 Fast Flow were from GE Healthcare; β-mercaptoethanol and glycerol were from Thermo Fisher Scientific; Bio-beads SM2 were from BIO-RAD; BSA and protease inhibitor cocktail were from Roche; non-fat milk powder was from CST; Accudenz was from Axell; trypsin was from Thermo Fisher Scientific; cy3 and cy5 were from Cytiva. The antibodies used in this study are listed in Table S2.

Table S2 List of antibodies used. The antibodies used in our experiments with the manufacturer details and the dilutions used are mentioned.