Fgf 21 quantikine elisa kit

Blood samples were collected from mice under anesthesia. Plasma was obtained after centrifugation (15 min, 845 ×g, 4°C) and concentrations of urea nitrogen, creatinine, cystatin C, glucose, total cholesterol, triglycerides, free fatty acids, phospholipids, FGF21 were measured using the BUN-Test-Wako (Wako, 279–36201), the CRE-EN Kainos (Kainos, TKA7500), cystatin C (mouse) ELISA kit (BioVendor, RD291009200R), the Glucose CII-test (Wako, 439–90901), the Cholesterol E-test (Wako, 439–17501), the Triglyceride E-test (Wako, 432–40201), the NEFA C-test (Wako, 279–75401), the Phospholipid C-test (Wako, 433–36201), and the Mouse/Rat FGF21 Quantikine ELISA Kit (R&D Systems, MF2100). Urinary albumin excretion was measured with the Microfluoral^TM microalbumin test (Progen, PR2005). All kits were used in accordance with the manufacturer’s protocols.

Blood samples were collected from mice under anesthesia. Plasma was obtained after centrifugation (15 min, 845 ×g, 4°C) and concentrations of urea nitrogen, creatinine, cystatin C, glucose, total cholesterol, triglycerides, free fatty acids, phospholipids, FGF21 were measured using the BUN-Test-Wako (Wako, 279–36201), the CRE-EN Kainos (Kainos, TKA7500), cystatin C (mouse) ELISA kit (BioVendor, RD291009200R), the Glucose CII-test (Wako, 439–90901), the Cholesterol E-test (Wako, 439–17501), the Triglyceride E-test (Wako, 432–40201), the NEFA C-test (Wako, 279–75401), the Phospholipid C-test (Wako, 433–36201), and the Mouse/Rat FGF21 Quantikine ELISA Kit (R&D Systems, MF2100). Urinary albumin excretion was measured with the Microfluoral^TM microalbumin test (Progen, PR2005). All kits were used in accordance with the manufacturer’s protocols.

Blood tests were performed on all subjects after 12 hr of fasting. The blood was drawn at the same time under the same conditions before and after the exercise. A disposable needle was used to draw 5 mL of venous blood from the anterior cardinal vein. The blood was stored in serum separating tubes for serum and centrifuged for 10 min at 3,000 rpm; 200 μL of the serum was stored in a sample tube. The irisin and FGF-21 levels were assessed using the enzyme linked immunosorbent assay (ELISA) method with the irisin ELISA kit (BioVendor, Brno, Czech Republic) and FGF-21 Quantikine ELISA kit (R&D System, Minneapolis, MN, USA), respectively.

Blood was collected from the tail vein and centrifuged at 5,000 g for 10 min at 4 °C. Serum Fgf21 levels were determined by a FGF-21 Quantikine ELISA kit (R&D Systems).