Fibrinogen, coagulation factors V and VIII (FV and FVIII), vWF:Ag, protein S and C activities, and D-dimer were measured using the BCS XP system (Siemens). Thrombin-antithrombin III complex (TAT), plasmin-α2-antiplasmin complex (PAP), tissue-type plasminogen activator (tPA) were all determined by ELISA assays (ASSERACHROM t-PA kit, Diagnostica Stago, S.A.S., France, Enzygnost PAP micro, and Enzygnost TAT micro, Siemens). Thrombin activatable fibrinolysis inhibitor (TAFI) was determined for protein content by ELISA (Imuclone Total TAFI ELISA, Sekisui Diagnostics LLC, USA) and for TAFI activity by kinetic assay (Sekisui Diagnostics GmbH, Germany). Plasminogen activator inhibitor levels (PAI-1) were determined by ELISA and measured both complexed and free PAI-1 (Zymutest PAI-1 Antigen, Hyphen BioMed, France).
Zymutest pai 1 antigen
Manufactured by Hyphen Biomed
Sourced in France
The Zymutest PAI-1 Antigen is a laboratory test kit used to measure the levels of plasminogen activator inhibitor-1 (PAI-1) antigen in biological samples. PAI-1 is a serine protease inhibitor that plays a role in the regulation of fibrinolysis, the process of dissolving blood clots. The Zymutest PAI-1 Antigen kit provides a quantitative determination of PAI-1 antigen levels, which can be useful in the assessment of various cardiovascular and thrombotic disorders.
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Lab products found in correlation
Enzygnost tat micro, by Siemens (1 mentions)
Bcs xp system, by Siemens (1 mentions)
Zymutest pai 1 activity, by Hyphen Biomed (1 mentions)
Acl top analyzer, by Werfen (1 mentions)
Nephelometry, by Siemens (1 mentions)
Zymutest t pa antigen, by Hyphen Biomed (1 mentions)
Human se selectin cd62e quantikine elisa kit, by R&D Systems (1 mentions)
Human thrombomodulin bdca 3 quantikine elisa kit, by R&D Systems (1 mentions)
Hemosil von willebrand factor antigen, by Werfen (1 mentions)
4 protocols using zymutest pai 1 antigen
1
Coagulation and Fibrinolysis Biomarkers Assay
2
Plasma Biomarker Measurement Protocol
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Platelet poor plasma was obtained by centrifugation of citrated blood at 2500 g for 20 min at room temperature. Aliquots of citrated plasma, 1 ml each, were stored, shortly after collection, at -80°C until PAI-1 antigen, PAI-1 activity, TAFI assay were performed.
D-dimer concentrations were measured in citrated plasma by the d-dimer HS kit on ACL TOP analyzer (Instrumentation Laboratory, IL, USA). PAI-1 antigen was analyzed in duplicate by the ELISA kit ZYMUTEST PAI-1 Antigen (HYPHEN Biomed France). PAI-1 activity was measured in duplicate by the ELISA kit ZYMUTEST PAI-1 Activity (HYPHEN Biomed France). TAFI antigen was analyzed in duplicate by the IMUCLONE TAFI ELISA (Sekisui Diagnostics USA).
D-dimer concentrations were measured in citrated plasma by the d-dimer HS kit on ACL TOP analyzer (Instrumentation Laboratory, IL, USA). PAI-1 antigen was analyzed in duplicate by the ELISA kit ZYMUTEST PAI-1 Antigen (HYPHEN Biomed France). PAI-1 activity was measured in duplicate by the ELISA kit ZYMUTEST PAI-1 Activity (HYPHEN Biomed France). TAFI antigen was analyzed in duplicate by the IMUCLONE TAFI ELISA (Sekisui Diagnostics USA).
3
Biomarkers in Fasting Blood Samples
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In fasting venous blood sample complete blood counts, plasma lipid profile, glucose, and creatinine were assayed by standard laboratory techniques. High-sensitive C-reactive protein and fibrinogen were assessed by nephelometry (Siemens, Marburg, Germany). Enzyme-linked immunosorbent assays were used to determine plasminogen activator inhibitor-1 (PAI-1) antigen (Zymutest PAI-1 Antigen, Hyphen BioMed, Neuville-Sur-Oise, France) and thrombin-activatable fibrinolysis inhibitor (TAFI) zymogen (Zymutest Activable TAFI, Hyphen BioMed), which is presented as a percentage of pooled standard plasma. 8-iso-prostaglandin F2α (8-iso-PGF2α) was assessed immunoenzymatically (Cayman Chemicals, Ann Arbor, MI, US).
4
Plasma Biomarkers of Endothelial Function
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Plasma levels of von Willebrand factor (vWF) antigen were measured using a commercial method (HemosIL Von Willebrand Factor Antigen, Instrumentation Laboratory, Bedford, MA, USA) with intra- and inter-assay CVs of <2.3%; the lower detection limit was 2.2%.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
Tissue-type plasminogen activator (t-PA) antigen was measured in plasma using a commercially available ELISA (Zymutest t-PA antigen, Hyphen BioMed, Neuville sur Oise, France) in accordance with the manufacturer's instructions. The intra- and inter-assay CVs were <10%, and the lower detection limit was 0.5 ng/mL.
Plasminogen activator inhibitor-1 (PAI-1) antigen was measured in plasma using a commercially available ELISA (Zymutest PAI-1 antigen, Hyphen BioMed) whose intra- and inter-assay CVs were 8% and 13%, respectively; the lower detection limit was 0.5 ng/mL.
Soluble plasma thrombomodulin (sTM) plasma levels were measured using a commercial sandwich ELISA (Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA) whose intra- and inter-assay CVs were both <10%; the lower detection limit was 7.82 pg/mL.
Soluble endothelial selectin (sE-selectin) was measured in plasma using a sandwich ELISA (Human sE-Selectin/CD62E Quantikine ELISA Kit, R&D Systems Inc.) whose intra- and inter-assay CVs were respectively 5% and 8.8%; the lower detection limit was 0.009 ng/mL.
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