Mouse breeder diet 5021

The Origins of Bone and Cartilage Disease (OBCD) programme⁶⁶ is undertaking a validated rapid-throughput multiparameter skeletal phenotype screen of mutant mouse lines generated by the Wellcome Trust Sanger Institute as part of the International Mouse Phenotyping Consortium (IMPC)^{111 (link)} and International Mouse Knockout Consortium (IMKC)¹¹² effort. The OBCD mouse studies were undertaken by Wellcome Trust Sanger Institute Mouse Genetics Project (MGP) as part of the IMPC. This was licensed by the UK Home Office (PPLs 80/2485 and P77453634) in accordance with the 1986 Animals (Scientific Procedures) Act and approved by the Wellcome Sanger Institute’s Animal Welfare and Ethical Review Body.
All mice generated by the MGP were produced (Tm1 alleles^{113 (link)}, CRISPR alleles^{114 (link)}) and maintained on a C57BL/6NTac background. Mice were fed either a Breeder’s Chow (Mouse Breeder Diet 5021, 21% kcal as fat, Labdiet, London, UK) or a high fat diet (Western RD, 82,9100, Special Diet Services, Witham, UK) from 4 weeks of age. Prior to tissue collection, mice underwent a standardised broad primary phenotype screen (https://www.mousephenotype.org/impress/PipelineInfo?id=15)^{115 (link)}. Detailed OBCD phenotyping methods were then performed as below:

Samples from 16-week old male WT and genetically modified mice designed with deletion alleles on the C57BL/6 N Taconic;C57BL/6 N background were generated as part of the Wellcome Trust Sanger Institute’s (WTSI) Mouse Genetics Project, part of the IMPC (http://www.mousephenotype.org). Details on back-crossing status, health status, administered drug and procedures, husbandry and specific conditions (including housing, food, temperature, and cage conditions) have been reported previously^{102 (link),103 (link)} with the exception that temperature was 21 ± 2 °C, humidity was 55 ± 10%, the diet used was Mouse Breeder Diet 5021 (Labdiet, London, UK) and the IMPReSS screen had four fewer tests (hair phenotyping, open field, hot plate, and stress-induced hypothermia). Further details available on request. Mouse strains, genotypes, and Research Resource Identifiers are included in Supplementary Data 12. Samples from male mice were used for the OBCD joint phenotyping pipeline and samples from females were used for the OBCD bone-phenotyping pipeline. The rationale that justified this approach was that (i) the gold standard DMM surgical provocation model for osteoarthritis is 100% penetrant in male mice but has variable penetrance in females^{24 ,99} , and (ii) the ovariectomy provocation model for post-menopausal osteoporosis can only be performed in females.