Tris hcl ph

Manufactured by Thermo Fisher Scientific

Sourced in United States

Tris-HCl pH is a buffer solution that maintains a stable pH range. It is commonly used in various laboratory applications to control and maintain the desired pH level of reagents, samples, or reactions.

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Lab products found in correlation

Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions) Tween 20, by Merck Group (2 mentions) Digitonin, by Merck Group (1 mentions) Np 40, by Roche (1 mentions) Tris hcl ph7, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by New England Biolabs (1 mentions) Chaps, by Merck Group (1 mentions) Cacl2, by Avantor (1 mentions) Mgcl2, by Merck Group (1 mentions)

Close spelling variants of this product

Tris-HCl (104 citations) Tris-HCl pH 8.0 (23 citations) Tris-HCl buffer (pH 7.2) (4 citations) 1.0 M Tris-HCl (pH 7.0) buffer (2 citations)

2 protocols using tris hcl ph

Single-Nucleus ATAC-Seq Library Preparation

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Cells were collected in 1.5 mL centrifuge tube and added lysis buffer which consisted of 10 mM Tris-HCl pH7.5 (Thermo Fisher Scientific, USA), 10 mM NaCl (Thermo Fisher Scientific, USA), 3 mM MgCl₂ (Thermo Fisher Scientific, USA), 0.1% Tween-20 (Sigma, USA), 0.1% NP-40 (Roche, Switzerland), 0.01% Digitonin (Sigma, USA), and 1% bovine serum albumin (BSA, BBI, UK). Then the lysates were centrifuged at 500 g for 5 min at 4°C and the supernatant was discarded. The obtained nuclei were then washed three times with ATAC wash buffer (10 mM Tris-HCl pH7.5 (Thermo Fisher Scientific, USA), 10 mM NaCl (Thermo Fisher Scientific, USA), 3 mM MgCl₂ (Thermo Fisher Scientific, USA), 0.1% Tween-20 (Sigma, USA), 1% bovine serum albumin (BSA, BBI, UK)). Nuclei were then transposed using transposase (BGI, China) and were resuspended in nuclear resuspension buffer. Transposed single-nucleus suspensions were converted to barcoded scATAC-seq libraries, through procedures including droplet encapsulation, pre-amplification, emulsion breakage, capture beads collection, DNA amplification and purification. The libraries were sequenced on the ultra-high-throughput DIPSEQ T1 sequencer with PE 50-bp read length.

Wang S., Xie J., Zou X., Pan T., Yu Q., Zhuang Z., Zhong Y., Zhao X., Wang Z., Li R., Lei Y., Yin J., Yuan Y., Wei X., Liu L., Liu S., Yang H, & Wu L. (2022). Single-cell multiomics reveals heterogeneous cell states linked to metastatic potential in liver cancer cell lines. iScience, 25(3), 103857.

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Preparation of Specialized Buffers

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A 2× stock of salt-Tris solution (ST buffer) containing 292 mM NaCl (Thermo Fisher Scientific, cat. no. AM9759), 20 mM Tris-HCl pH 7.5 (Thermo Fisher Scientific, cat. no. 15567027), 2 mM CaCl₂ (VWR International Ltd, cat. no. 97062-820) and 42 mM MgCl₂ (Sigma Aldrich, cat. no. M1028) in ultrapure water was made and used to prepare three buffers: for CST, 1 ml of 2× ST buffer, 980 µl of 1% CHAPS (Millipore, cat. no. 220201), 10 µl of 2% BSA (New England BioLabs, cat. no. B9000S) and 10 µl of nuclease-free water; for TST, 1 ml of 2× ST buffer, 60 µl of 1% Tween-20 (Sigma Aldrich, cat. no. P-7949), 10 µl of 2% BSA (New England Biolabs, cat. no. B9000S) and 930 µl of nuclease-free water; for NST, 1 ml of 2× ST buffer, 40 µl of 10% Nonidet P40 Substitute (Fisher Scientific, cat. no. AAJ19628AP), 10 µl of 2% BSA (NEB) and 950 µl of nuclease-free water. 1× ST buffer was prepared by dilution 2× ST with ultrapure water (Thermo Fisher Scientific cat. no. 10977023) in a ratio of 1:1.

Slyper M., Porter C.B., Ashenberg O., Waldman J., Drokhlyansky E., Wakiro I., Smillie C., Smith-Rosario G., Wu J., Dionne D., Vigneau S., Jané-Valbuena J., Tickle T.L., Napolitano S., Su M.J., Patel A.G., Karlstrom A., Gritsch S., Nomura M., Waghray A., Gohil S.H., Tsankov A.M., Jerby-Arnon L., Cohen O., Klughammer J., Rosen Y., Gould J., Nguyen L., Hofree M., Tramontozzi P.J., Li B., Wu C.J., Izar B., Haq R., Hodi F.S., Yoon C.H., Hata A.N., Baker S.J., Suvà M.L., Bueno R., Stover E.H., Clay M.R., Dyer M.A., Collins N.B., Matulonis U.A., Wagle N., Johnson B.E., Rotem A., Rozenblatt-Rosen O, & Regev A. (2020). A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors. Nature Medicine, 26(5), 792-802.

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