To measure expressions of surface molecules, cells were stained with FITC anti-mouse major histocompatibility complex (MHC)-II antibody (1:200; Biolegend, 107606), BV510 anti-mouse CD3 antibody (1:200; Biolegend, 100234), FITC anti-mouse CD4 antibody (1:200; Biolegend, 100405) or APC anti-mouse CD25 antibody (1:200; Biolegend, 101909) in FACS buffer for 40 min. For intracellular staining, cells were permeabilized, followed by fixation using the Foxp3 staining buffer kit prior to staining with PE anti-mouse Foxp3 antibody (1:100; Biolegend, 320007), BV421 anti-mouse Foxp3 antibody (1:100; Biolegend, 126419), or PE-conjugated S100A9 antibody (1:100; Cell Signaling Technology, 93941). Flow cytometry analyses were performed on a LSR II instrument and raw data were retrieved. Results were analyzed by use of FlowJo software version 10.0.
Bv421 anti mouse foxp3 antibody
Manufactured by BioLegend
Sourced in United States
The BV421 anti-mouse Foxp3 antibody is a fluorochrome-conjugated antibody that specifically binds to the Foxp3 transcription factor expressed in mouse regulatory T cells. It can be used for the identification and analysis of Foxp3-positive cells in flow cytometry applications.
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Lab products found in correlation
Pe anti mouse foxp3 antibody, by BioLegend (1 mentions)
Fitc anti mouse cd4 antibody, by BioLegend (1 mentions)
Apc anti mouse cd25 antibody, by BioLegend (1 mentions)
Apc anti mouse cd3 antibody, by BioLegend (1 mentions)
Bv510 anti mouse cd45 antibody, by BioLegend (1 mentions)
Fitc anti mouse cd80 antibody, by BioLegend (1 mentions)
Pe cy7 anti mouse f4 80 antibody, by BioLegend (1 mentions)
Pe anti mouse cd206 antibody, by BioLegend (1 mentions)
Cd16 32, by BioLegend (1 mentions)
2 protocols using bv421 anti mouse foxp3 antibody
1
Multiparametric Flow Cytometry Analysis
2
Comprehensive Flow Cytometry Antibody Panel
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The following flow cytometry antibodies were purchased from BioLegend company (San Diego, USA): CD16/32 (clone: 93), BV510 anti-mouse CD45 antibody (clone: 30-F11), BV711 anti-mouse CD8a antibody (clone: 53-6.7), APC anti-mouse CD3 antibody (clone: 17A2), BV421 anti-mouse Foxp3 antibody (clone: MF-14), PE anti-mouse CD206 antibody (clone: C068C2), BV605 anti-mouse CD11b antibody (clone: M1/70), PE/Cy7 anti-mouse F4/80 antibody (clone: QA17A29), BV650 anti-mouse CD25 antibody (clone: PC61), Percp/Cy5.5 anti-mouse Gr-1 (Ly-6G/Ly-6C) antibody (clone: RB6-8C5), APC/Cy7 anti-mouse CD86 antibody (clone: GL-1), FITC anti-mouse CD80 antibody (clone: 16-10A1), PE/Cy5 anti-mouse CD19 (clone: 6D5), and Alexa fluor 700 anti-mouse MHC class II antibody (clone: M5/114.15.2). Flow cytometry antibodies for BUV563 anti-mouse CD4 (clone: RM4-5) was purchased from BD Company (New York, USA).
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