Fc 3283

Manufactured by 3M

Sourced in United States

FC-3283 is a laboratory equipment product from 3M. It is designed for use in research and scientific applications. The core function of this product is to provide a reliable and consistent measurement or analysis tool for laboratory environments. No further details or extrapolation on the intended use of this product are provided.

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Lab products found in correlation

Fvbtg gadgfp 45704swn j, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Fluorinert (FC-3283,

5 protocols using fc 3283

High-Contrast Postmortem Brain Imaging

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Brain samples were placed in a container with Fluorinert FC3283 (3M) and then degassed before being placed in a human head coil. Six to eight hemispheres were scanned simultaneously.
In postmortem brains, conventional T₁-weighted structural protocols did not produce satisfying gray/white matter contrast; therefore, we used a three-dimensional balanced SSFP (steady-state free precession) pulse sequence previously adapted for scanning formalin-preserved postmortem samples (65 (link)). The TRUFI (true fast imaging with steady-state free precession) sequence chosen produced high gray/white matter contrast, albeit inverted compared with conventional T₁-weighted structural acquisitions (gray matter has high relative signal; white matter, low).

Testard C., Brent L.J., Andersson J., Chiou K.L., Negron-Del Valle J.E., DeCasien A.R., Acevedo-Ithier A., Stock M.K., Antón S.C., Gonzalez O., Walker C.S., Foxley S., Compo N.R., Bauman S., Ruiz-Lambides A.V., Martinez M.I., Skene J.H., Horvath J.E., Unit C.B., Higham J.P., Miller K.L., Snyder-Mackler N., Montague M.J., Platt M.L, & Sallet J. (2022). Social connections predict brain structure in a multidimensional free-ranging primate society. Science Advances, 8(15), eabl5794.

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Perfused Mouse Brain Tissue Preparation

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Perfusion‐fixed brain tissue samples were obtained from four adult male mice (approximate age, postnatal day 140; FVB‐Tg (GadGFP) 45704 Swn/J; stock #003718; Jackson Laboratory, Bar Harbor, Maine, USA). Animals were treated according to national guidelines and institutional oversight. Brain specimens were stored in 4% paraformaldehyde solution for 48 hours and then transferred to a phosphate‐buffered saline solution for rehydration and storage. Prior to imaging, each specimen was transferred to a 10‐mm‐diameter NMR tube system (Shigemi Inc., Allison Park, Pennsylvania, USA) and immersed in fluorinert (FC‐3283; 3M, St. Paul, Minnesota, USA).

Hutchinson E.B., Avram A.V., Irfanoglu M.O., Koay C.G., Barnett A.S., Komlosh M.E., Özarslan E., Schwerin S.C., Juliano S.L, & Pierpaoli C. (2017). Analysis of the effects of noise, DWI sampling, and value of assumed parameters in diffusion MRI models. Magnetic Resonance in Medicine, 78(5), 1767-1780.

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Fluorosurfactant Synthesis and Application

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Example 9

Fluorosurfactants are synthesized by reacting Krytox 157 FSL, FSM, or FSH with aqueous ammonium hydroxide in a volatile fluorinated solvent. The solvent and residual water and ammonia are removed with a rotary evaporator. The surfactant can then be dissolved in a fluorinated oil (e.g., FC-3283 from 3M), which can then be used as the continuous phase of the emulsion. A typical concentration is 2.5 wt % of surfactant dissolved in the oil.

The channels of the microfluidic device are also coated with a fluorinated surface product. For example, the coating is applied from a 0.1-0.5 wt % solution of Cytop CTL-809M in CT-SoIv 180. This solution is injected into the channels of a microfluidic device via a plastic syringe. The device is then heated to 90° C. for two hours, followed by 200° C. for an additional two hours. These surfactants in the fluorinated oil stabilize the aqueous droplets from spontaneously coalescing. By fluorinating the channel surfaces, the oil phase preferentially wets the channels and allows for the stable generation and movement of droplets through the device, the low surface tension of the channel walls minimizes the accumulation of channel clogging particulates.

US10633652B2. Microfluidic devices and methods of use in the formation and control of nanoreactors (2020-04-28). Bio-Rad Laboratories, Inc. [US]. Inventors: Darren Roy Link [US], Laurent Boitard [US], Jeffrey Branciforte [US], Yves Charles [US], Gilbert Feke [US], John Q. Lu [US], David Marran [US], Ahmadali Tabatabai [US], Michael Weiner [US], Wolfgang Hinz [US], Jonathan M. Rothberg [US].

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Microfluidic Chip Fabrication Protocol

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Microfluidic chips were hybrid PDMS/glass chips fabricated using soft-lithography [28 (link),29 (link)] by the University of Utah microfluidics foundry. The designs (File S1) were prepared with AutoCAD software and printed at 25,400 dpi resolution onto a Fuji transparency mask (CAD/Art Services Inc., Bandon, OR, USA). Upon reception, the microfluidic chips were treated with a fluorinated trichloro silane reagent (heptadecafluoro-1,1,2,2-tetrahydrodecyl) trichlorosilane-CF₃-(CF₂)₆-CH₂-CH₂-SiCl₃, Gelest, PA, USA) diluted at 1% weight in HFE7500 oil (3M) [28 (link)]. The solution was injected into channels with a disposable syringe, through a hydrophobic 0.2 μm disc filter and a blunt needle, and flushed out with FC 3283 (3M, Maplewood, MN, USA) oil after 5 min of incubation. We injected liquid low-melting solder (Cerrolow-117, 47 °C melting temperature, Bolton Metal Products, Bellefonte, PA, USA) in dedicated channels while the chip was incubated on a hot plate to fabricate the electrodes. A piece of copper foil tape with conductive adhesive was affixed to the back of the chip to confine the effects of the electric field.

Theodorou E., Scanga R., Twardowski M., Snyder M.P, & Brouzes E. (2017). A Droplet Microfluidics Based Platform for Mining Metagenomic Libraries for Natural Compounds. Micromachines, 8(8), 230.

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Brain Tissue Preparation for MRI Imaging

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After animal sacrifice, the neck was incised, jugular veins transected, and angiocatheters were placed in the bilateral carotid arteries. The carotid arteries were then each flushed with 60 mL phosphate-buffered saline (PBS) + ethylenediaminetetraacetic acid (EDTA) to clear the cerebral vasculature of blood, followed by 60 mL 10% formalin for fixation. Brains were then harvested intact via craniotomy and placed in 10% formalin.
Prior to imaging, the brains were removed from formalin and soaked in 0.05% sodium azide and 1 equivalent PBS at room temperature. This solution was changed daily for 3–4 weeks prior to MRI.[13 (link), 14 ] For each MRI experiment, the brain was immersed in Fluorinert, a ¹H proton-free, perflurocarbon that improves signal-to-noise ratio in explanted tissue MRI (FC-3283, 3M Company: Maplewood, MN).[15 (link)]

Church J.T., Werner N.L., Coughlin M.A., Menzel-Smith J., Najjar M., Carr B.D., Parmar H., Neil J., Alexopoulos D., Perez-Torres C., Ge X., Beeman S.C., Garbow J.R, & Mychaliska G.B. (2018). Effects of an Artificial Placenta on Brain Development and Injury in Premature Lambs. Journal of pediatric surgery, 53(6), 1234-1239.

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