Sf9, VE-CL02, High Five, VE-High Five, SfSWT-4, and VE-SWT cells were infected with YFP-AcMNPV at an MOI of 5 in triplicate. On days 2–5 post-infection, photomicrographs and YFP fluorescence (300 ms exposure time) for each infection well were captured using a Zeiss AX10 inverted fluorescence microscope with a 20× objective and the AxioVision Rel. 4.6 program Carl Zeiss Microscopy). After gently collecting the cells, viability was determined by staining cells with trypan blue (Thermo Fisher Scientific) and counting viable and non-viable cells using an improved Neubauer hemocytometer under magnification of a Zeiss AX10 inverted microscope Carl Zeiss Microscopy). To quantify YFP fluorescence, insect cells were first counted in triplicate utilizing a Guava® easyCyte HT Sampling Flow Cytometer and the guava InCyte Assay software module (EMD Millipore, Hayward, CA, USA). YFP fluorescence was then measured using a 405nm laser at a green spectral imaging band (525/30nm) of the Guava® easyCyte™ HT Sampling Flow Cytometer. The total fluorescence of each infection was determined with the Guava® InCyte Assay software module.
Guava incyte assay software module
Manufactured by Merck Group
Sourced in United States
The Guava InCyte Assay software module is a laboratory equipment product from Merck Group. It serves as a tool for analyzing and interpreting data from flow cytometry experiments.
Automatically generated - may contain errors
2 protocols using guava incyte assay software module
1
Quantifying Virus Infection and Cell Viability
2
Baculovirus Infection Kinetics in Insect Cells
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Sf9, VE‐CL02, High Five, VE‐High Five, SfSWT‐4, and VE‐SWT cells were infected with YFP‐AcMNPV at an MOI of 5 in triplicate. On days 2–5 post‐infection, photomicrographs and YFP fluorescence (300 ms exposure time) for each infection well were captured using a Zeiss AX10 inverted fluorescence microscope with a 20× objective and the AxioVision Rel. 4.6 program (Carl Zeiss Microscopy). After gently collecting the cells, viability was determined by staining cells with trypan blue (Thermo Fisher Scientific) and counting viable and non‐viable cells using an improved Neubauer hemocytometer under magnification of a Zeiss AX10 inverted microscope (Carl Zeiss Microscopy). To quantify YFP fluorescence, insect cells were first counted in triplicate utilizing a Guava® easyCyte HT Sampling Flow Cytometer and the guava InCyte Assay software module (EMD Millipore, Hayward, CA). YFP fluorescence was then measured using a 405‐nm laser at a green spectral imaging band (525/30 nm) of the Guava® easyCyte™ HT Sampling Flow Cytometer. The total fluorescence of each infection was determined with the Guava® InCyte Assay software module.
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