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Cd27 apc efluor780 o323

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CD27 APC-eFluor780 (O323) is a fluorescently labeled antibody that binds to the CD27 receptor on the surface of T cells. It can be used for the detection and analysis of CD27-positive cells in flow cytometry applications.

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Lab products found in correlation

Cellsdirect 2x reaction mix, by Thermo Fisher Scientific (2 mentions) Cd3 alexa 700 sp34 2, by BD (2 mentions) Cd14 qdot605 tuk4, by Thermo Fisher Scientific (2 mentions) Hla dr pe cy7 g46 6, by BD (2 mentions) Cd45ra ecd 3p, by Beckman Coulter (2 mentions) Cd8 pe sk1, by BD (2 mentions) Amine aqua amcyan, by Thermo Fisher Scientific (2 mentions) Rnaqueous micro lysis buffer, by Thermo Fisher Scientific (2 mentions) Cd16 fitc 3g8, by BD (2 mentions) Cd4 qdot655 s3, by Thermo Fisher Scientific (2 mentions) Facsaria, by BD (2 mentions)

Close spelling variants of this product

CD27-APC-eFluor780 CD27 (O323) CD27-APC

2 protocols using cd27 apc efluor780 o323

1

Multicolor Flow Cytometry Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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2

Multicolor Flow Cytometry Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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