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Glyburide

Manufactured by MedChemExpress
Sourced in United States

Glyburide is a pharmaceutical compound used as a laboratory reagent. It is a sulfonamide drug primarily used to treat type 2 diabetes by stimulating the release of insulin from the pancreas. Glyburide functions as an antidiabetic agent in biochemical research and development applications.

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2 protocols using glyburide

1

Aeromonas caviae Immune Response Analysis

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Aeromonas caviae (ATCC 15,468) was kept in our lab. The V X765 (specific caspase-1 inhibitor), CA074-Me (specific cathepsin B inhibitor) and BAY11–7082 (specific NF-κB inhibitor) were all obtained from Selleck (Shanghai). The Glyburide (specific K+ efflux inhibitor) and MCC950 (effective selective inhibitor of NLRP3) were aquired from MedChemExpress (Monmouth Junction). The gentamicin was obtained from Biological Industries (Israel). Antibodies of NLRP3 and caspase-1 were from Adipogen (San Diego). IL-1β was from R&D (Minneapolis). ASC was from Proteintech (Wuhan). The uncoated commercial kits for measuring mouse TNF-α, IL-6 and IL-1β were from Invitrogen (California).
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2

NLRP3 Inflammasome Activation Assay

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V. alginolyticus ATCC 17749 was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Caspase-1 (p20, AG-20B-0042) and NLRP3 (AG-20B-0014) were purchased from AdipoGen Life Sciences (Liestal, Switzerland). IL-1β (p17) was purchased from R&D (AF-401-SP, Minnesota, USA). Ac-YVAD-CHO was purchased from Enzo Life Sciences (ALX-260-027, Lausen, Switzerland). Z-VAD-FMK and CA-074 methyl ester were obtained from Selleck (S7023, S7042, Shanghai, China). Glyburide was obtained from MedChemExpress (HY-15206, St. New Jersey, USA). RIPA was purchased from Solarbio (R0020, Beijing, China). RPMI 1640 (01-100-1ACS), fetal bovine serum (FBS, 04-001-1ACS), and gentamicin (03-035-1C) were all obtained from Biological Industries (Israel). The TRIzol reagent was purchased from Monad (MI20101S, Wuhan, China). The LDH Cytotoxicity Assay Kit was obtained from Beyotime (C0016, Shanghai, China).
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