Ab99887

Fresh, matched CRC tissues and adjacent normal tissues used for biological analyses and organoid establishment were obtained from Jiangsu Province Hospital of Chinese Medicine (Nanjing, China). All patients gave written informed consent for participation in this study. The study on fresh clinical samples was approved by the ethics committee of Jiangsu Province Hospital of Chinese Medicine.
IHC was performed by Nanjing Microworld Biotechnology Co., Ltd. using paraffin-embedded, formalin-fixed tumor tissues removed from the tumor-bearing nude mice at the end of the study. Sections were incubated with antibodies specific for PRMT1 (Proteintech, 11279–1, 1:100), H4R3me2a (PTMBio, PTM667, 1:100), EGFR (Abcam, ab52894, 1:200), TNS4 (Abcam, ab99887, 1:200) and SMARCA4 (Abcam, ab110641, 1:100). Immunocomplexes were visualized using a 2-Solution DAB Kit (Invitrogen).

Cellular proteins were extracted using cell lysis buffer for Western blots (P0013, Beyotime). All proteins were separated by SDS–PAGE, followed by semidry electroblotting onto PVDF membranes (Roche). Primary antibodies against SMARCA4 (ab110641, 1:1000, Abcam), Flag tag (AE005, 1:1000, Abclonal), EGFR (ab52894, 1:1000, Abcam), TNS4 (ab99887, 1:1000, Abcam), GST tag (AE001, 1:1000, Abclonal), SNF5 (#191735, 1:1000, Cell Signaling Technology), ARID1A (#12354, 1:1000, Cell Signaling Technology), ARID1B (#92964, 1:1000, Cell Signaling Technology), BAF57 (#33360, 1:1000, Cell Signaling Technology), and GAPDH (A19056, 1:1000, Abclonal), PBRM1 (ab305223, 1:1000, Abcam), ARID2 (ab245529, 1:1000, Abcam), BRD7 (ab270982, 1:1000, Abcam) and BRD9 (ab259839, 1:1000, Abcam) were used. HRP-conjugated goat anti-rabbit IgG (AS014, 1:10,000, Abclonal) and goat anti-mouse IgG (AS003, 1:10,000, Abclonal) were used as the secondary antibodies. Blots were exposed to X-ray film after development using High-sig Western ECL Blotting Substrate (Tanon, Shanghai), and the films were scanned as image files using an Epson Perfection V700 Photo Scanner.). All western blots within a panel are from the same experiment and all blots were processed in parallel. For uncropped images and scans of the blots, refer to Supplementary Figs. 8–18.