Nahco3

Manufactured by Kanto Chemical

Sourced in Japan

NaHCO3 is a chemical compound commonly known as sodium bicarbonate. It is a white, crystalline solid that is soluble in water. The core function of NaHCO3 is to serve as a pH buffer, helping to maintain a stable pH level in various applications.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using nahco3

Recombinant Protein Production in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

BP1 was produced by a fermentation process using E.coli, which was previously reported in the literature^{28 ,44} and provided in an unprocessed powder form by Spiber Inc. The amino acid composition of BP1 was alanine (16.4%), tyrosine (12.0%), glutamine (23.7%), glycine (21.9%), proline (15.3%), serine (9.3%) and others (1.4%). The protein sequence structure is shown in Fig. 1.
KH₂PO₄, K₂HPO₄, NaCl, Na₂HPO₄·H₂O, NH₄Cl, MgCl₂·6H₂O, CaCl₂, FeCl₃·6H₂O, Yeast extract, and agar powder were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Na₂SO₄, KCl, HCl, Na₂SO₄, NaHCO₃, and NaOH were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Plysurf was purchased from DKS Co. Ltd. (Kyoto, Japan). All chemicals were of reagent grade and used without further purification. Pronase E (P5147 Protease Type XIV from Streptomyces griseus) was purchased from Sigma Aldrich, Proteinase K was purchased from TaKaRa Bio Inc (Kusatsu, Japan), and chymotrypsin was purchased from NACALAI TESQUE, INC (Kyoto, Japan).

Tachibana Y., Darbe S., Hayashi S., Kudasheva A., Misawa H., Shibata Y, & Kasuya K.I. (2021). Environmental biodegradability of recombinant structural protein. Scientific Reports, 11, 242.

+ Open protocol

+ Expand

Porcine Collagen Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellmatrix Type I-A (3 mg/mL, pH 3, collagen derived from porcine tendons by acid extraction) was ordered from Nitta Gelatin Co., Ltd. (Osaka, Japan). Collagen buffer was prepared in accordance with the manufacture's protocol. Briefly, in 100 mL 0.05 N NaOH, 2.2 g NaHCO₃ (262 mM final) and 4.77 g HEPES (20 mM final) were dissolved. NaOH and NaHCO₃ were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan); HEPES and 10X Hank's, from Sigma-Aldrich Co., Ltd. (Saint Louis, MO, USA).

Pauty J., Usuba R., Takahashi H., Suehiro J., Fujisawa K., Yano K., Nishizawa T, & Matsunaga Y.T. (2017). A Vascular Permeability Assay Using an In Vitro Human Microvessel Model Mimicking the Inflammatory Condition. Nanotheranostics, 1(1), 103-113.

+ Open protocol

+ Expand

Isolation and Culture of Primary Rat Hepatocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary rat SHs were isolated by the collagenase perfusion method as previously described22 (link). Cells were suspended in serum-free Dulbecco’s modified Eagle’s medium/Nutrient Mixture Ham F-12 (DMEM/F12; Sigma-Aldrich Co., St.Louis, MO) supplemented with 20 mM HEPES (Dojindo Laboratories, Kumamoto, Japan), 25 mM NaHCO₃ (Kanto Chemical Co. Inc., Tokyo, Japan), 30 mg/L L-proline (Sigma-Aldrich Co.), 0.1% bovine serum albumin (BSA; Serologicals Proteons Inc., Kankakee, IL), 10 mM nicotinamide (Sigma-Aldrich Co.), 1 mM ascorbic acid-2 phosphate (Asc2P; Wako Pure Chem. City, Japan), 10⁻⁷ M dexamethasone (Dex; Wako Pure Chem.), 0.5 mg/L insulin-transferrin-selenium (ITS-X; GIBCO-BRL Invitrogen, Grand Island, NY), 10 ng/mL epidermal growth factor (EGF; BD Biosciences, San Jose, CA) and antibiotics. After the number of viable cells was counted using the trypan blue exclusion test, the cells (2.0 × 10⁴ cells/cm²) were plated on 35-, 60- or 100-mm dishes (Corning Inc., Corning, NY) coated with 1 mg/L HA (Sigma-Aldrich Co.). Three hours after plating, the cells were washed with the medium, and the medium was replaced every other day.

Ishii M., Kino J., Ichinohe N., Tanimizu N., Ninomiya T., Suzuki H., Mizuguchi T., Hirata K, & Mitaka T. (2017). Hepatocytic parental progenitor cells of rat small hepatocytes maintain self-renewal capability after long-term culture. Scientific Reports, 7, 46177.

+ Open protocol

+ Expand

Calcium Carbonate Production from Calcium Silicate

Check if the same lab product or an alternative is used in the 5 most similar protocols

GLDA tetrasodium salt (GLDA-4Na, C₉H₉NNa₄O₈) solutions with different concentrations were prepared by mixing the initial GLDA-4Na solution (40 wt% in water, Tokyo Chemical Industry, Japan) with Milli-Q water, and pH was adjusted from an initial value of 13.8–~9.0 through the addition of aqueous HNO₃ (60–61%, Kanto Chemical, Japan). This weakly alkaline pH was used at the beginning of the Ca extraction process to minimize the cost of pH regulation, as subsequent CaCO₃ precipitation requires alkaline environments (pH > 8)^{32 (link),33 (link)}. High-purity commercial calcium silicate powders (Fig. 2, < 30 µm) with a general composition of CaSiO₃ (Wako Pure Chemical Industries, Japan) were used as a Ca source to represent silicate minerals. Na₂CO₃ (>99.0%, Kanto Chemical, Japan), NaHCO₃ (> 99.0%, Kanto Chemical, Japan), and CO₂ gas (> 99.995 vol.%, Taiyo Nippon Sanso, Japan) were used as CO₂ sources to optimize the CaCO₃ production system.Figure 2

Representative X-ray diffraction (XRD) pattern of the employed calcium silicate.

Wang J., Watanabe N., Inomoto K., Kamitakahara M., Nakamura K., Komai T, & Tsuchiya N. (2021). Enhancement of aragonite mineralization with a chelating agent for CO2 storage and utilization at low to moderate temperatures. Scientific Reports, 11, 13956.

+ Open protocol

+ Expand

Ion Chromatography Analysis of Anions and Cations

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the extraction processes, the anion and cation concentrations of each fraction were measured by ion chromatography (IC), using the Metrohm 930 Compact IC Flex system (Metrohm AG, Herisau, Switzerland). For cations, the samples were eluted through a Metrohm Metrosep C6-250/4.0 column with 8 mM ultrapure HNO₃ (TAMAPURE AA-100, Tama Chemical, Kawasaki, Japan) at a flow rate of 0.9 mL·min⁻¹. Anions were measured with a Metrohm Metrosep A Supp4-250/4.0 column with a chemical suppressor module. The mobile phase consisted of a mixture of 1.8 mM Na₂CO₃ and 1.7 mM NaHCO₃ (Kanto Chemical, Tokyo, Japan) at a flow rate of 0.9 mL·min⁻¹. A chemical suppressor module (Metrohm MSM) was used to decrease the background conductivity of the eluent and to transform the analytes into free anions. The column temperature was set at 35 °C throughout the analysis. Detection of cations and anions was accomplished by measuring electrical conductivity.

Yoshimura T., Takano Y., Naraoka H., Koga T., Araoka D., Ogawa N.O., Schmitt-Kopplin P., Hertkorn N., Oba Y., Dworkin J.P., Aponte J.C., Yoshikawa T., Tanaka S., Ohkouchi N., Hashiguchi M., McLain H., Parker E.T., Sakai S., Yamaguchi M., Suzuki T., Yokoyama T., Yurimoto H., Nakamura T., Noguchi T., Okazaki R., Yabuta H., Sakamoto K., Yada T., Nishimura M., Nakato A., Miyazaki A., Yogata K., Abe M., Okada T., Usui T., Yoshikawa M., Saiki T., Tanaka S., Terui F., Nakazawa S., Watanabe S.I., Tsuda Y, & Tachibana S. (2023). Chemical evolution of primordial salts and organic sulfur molecules in the asteroid 162173 Ryugu. Nature Communications, 14, 5284.

+ Open protocol

+ Expand

Investigating NDMA Formation in RO Membranes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analytical grade chemicals (NaCl, NaHCO3, CaCl2, NH4Cl, and HCl) were purchased from Kanto Chemical Co. (Tokyo, Japan). A NaOCl solution, with over 12% chlorine concentration, was purchased from Tosoh Co. (Tokyo, Japan), and a stock solution of chloramine was prepared at 0.2 M by mixing NaOCl and NH4Cl solutions. Further, hypobromite stabilized by sulfamic acid was used as stabilized hypobromite in this study. A stock solution of stabilized hypobromite comprised of hypobromite, sulfamic acid and sodium hydroxide was supplied by Organo Co. (Tokyo, Japan).
Chemicals selected as NDMA precursors were DMA, TMA, and N,N-dimethylbenzylamine (DMBA) were purchased from Kanto Chemical Co. (Tokyo, Japan) (Table 1). A flat sheet polyamide composite RO membrane sample (namely ESPA2) were supplied by Hydranautics/Nitto (Oceanside, CA, USA). This study used two types of treated wastewater: an ultrafiltration (UF)-treated wastewater and membrane bioreactor (MBR)-treated wastewater, both of which were collected at wastewater treatment plants in Japan.

Fujioka T., Yoshikawa H., Eguchi M., Boivin S, & Kodamatani H. (2020). Application of stabilized hypobromite for controlling membrane fouling and N-nitrosodimethylamine formation. Chemosphere, 240.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!