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Rat igg1 hrpn

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Rat IgG1 (HRPN) is a laboratory reagent used for immunological assays and research applications. It functions as a secondary antibody that binds to primary rat IgG1 antibodies, allowing for their detection and quantification.

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Lab products found in correlation

Anti pd 1 ab clone j43 or rmp1 14, by BioXCell (2 mentions) Armenian hamster igg or igg2a mab clone 2a3, by BioXCell (2 mentions) Fastprep 24 5g homogenizer, by MP Biomedicals (2 mentions) Anti ifn γ ab clone xmg1, by BioXCell (2 mentions) Anti cd8β clone lyt 3, by BioXCell (1 mentions) Rat igg2a 2a3, by BioXCell (1 mentions) Mouse igg1 mopc 21, by BioXCell (1 mentions) Lps from escherichia coli strain 055 b5, by Merck Group (1 mentions) Lipopolysaccharides (lps), by BioXCell (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

6 protocols using rat igg1 hrpn

1

Modulating Antitumor Immunity in B16F10 Melanoma

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Mice were treated with either anti-LAP or IC mAbs prepared in PBS. Mouse anti-LAP monoclonal antibodies were isolated from hybridoma generated in-house. Two clones were employed for in vivo treatments: TW7-28G11 (IgG2b) and TW7-16B4 (IgG1). Respective ICs, MPC-11 (IgG2b) and MOPC-21 (IgG1), anti-CD103 (clone M290) and anti-PD1 (RMP-1) were purchased from BioXCell. As a standard treatment, antibodies were administered intraperitoneallly, 10 mg/kg every third day following tumor implantation. In some experiments, mice were treated i.p. with 100 μg per mouse of anti-CD8β (clone Lyt-3.2; BioXCell) Ab or IC (rat IgG1, HRPN; BioXCell) on days –1, 7, and 14 after B16F10 implantation.
Gabriely G., da Cunha A.P., Rezende R.M., Kenyon B., Madi A., Vandeventer T., Skillin N., Rubino S., Garo L., Mazzola M.A., Kolypetri P., Lanser A.J., Moreira T., Faria A.M., Lassmann H., Kuchroo V., Murugaiyan G, & Weiner H.L. (2017). Targeting latency-associated peptide promotes anti-tumor immunity. Science immunology, 2(11), eaaj1738.
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2

Inhibition of IFN-γ and IL-18 in Murine Infection

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Mice were injected with 500 μg of anti-IFN-γ (XMG1.2, BioXCell) or Rat IgG1 (HRPN, BioXCell) intraperitoneally at day 8, 10, 12, 14, 16 and 18 post infection. Mice were injected with 100 μg of anti-IL-18 (YIGIF74–1G7, BioxCell) or Rat IgG2a (2A3, BioxCell) intraperitoneally at day 9, 10, 11, 12 and 13 post infection.
Parsa R., London M., de Castro T.B., Reis B., des Amorie J.B., Smith J.G, & Mucida D. (2022). Newly recruited intraepithelial Ly6A+CCR9+CD4+ T cells protect against enteric viral infection. Immunity, 55(7), 1234-1249.e6.
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3

Antibody Administration for Colitis Alleviation

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Antibodies or corresponding isotype controls, diluted in sterile PBS, were administered intraperitoneally (ip) once weekly (0.5 mg/mouse/week) during the second and the third cycle of DSS administration (from day 21 until day 63). The PC 61 cell clone (Rat anti-mouse CD25 IgG1, #TIB-222) was obtained from ATCC (Manassas, VA)24 (link). Rat IgG1 HRPN (cat #BE0088), anti-mIFN-γ (XMG1.2, cat #BE0055) and mouse IgG1 (MOPC-21, cat #BE0083) were obtained from BioXCell (West Lebanon, NH)25 (link)–27 (link). Mouse anti-mIL-17A (MM17F3) and mouse anti-mIL-17F (MM17F-8FS) were generated and kindly provided by Jacques Van Snick and Catherine Uyttenhove (Ludwig Institute for Cancer Research, Brussels, Belgium)28 (link),29 (link). In control mice, not exposed to DSS, the corresponding volume of sterile PBS was administered.
Creyns B., Cremer J., Hoshino T., Geboes K., de Hertogh G., Ferrante M., Vermeire S., Ceuppens J.L., Van Assche G, & Breynaert C. (2019). Fibrogenesis in Chronic DSS Colitis is Not Influenced by Neutralisation of Regulatory T Cells, of Major T Helper Cytokines or Absence of IL-13. Scientific Reports, 9, 10064.
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4

MCMV Infection and Immunomodulation Protocol

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Stocks of Smith strain MCMV were generated by homogenizing salivary glands harvested from six-week-old BALB/c mice infected with 2.5 x 103 PFU of MCMV at the age of three weeks. Mice were infected at 2 p.m., by the ip injection of 103 PFU/g or 3 x 103 PFU/g (LD50) MCMV diluted in DMEM. “Non-infected” (NI) mice received DMEM only. For PD-1 blockade experiments, 250 μg anti-PD-1 Ab (clone J43 or RMP1-14) or the appropriate isotype control Ab (Armenian Hamster IgG or IgG2a mAb clone 2A3) (all from BioXCell) were injected i.p. into mice on day 1 PI. For IFN-γ neutralization, 500 μg anti-IFNγ Ab (clone XMG1.2) or Rat IgG1 (HRPN) (both from BioXCell) were injected i.p. into mice on day 1 PI. Spleens and livers were harvested after perfusion at different time points, and were processed for FACS or histology analysis, or weighed and homogenized for RNA or protein extraction. Organs were homogenized in a FastPrep-24™ 5G homogenizer (MP Biomedicals).
Quatrini L., Wieduwild E., Escaliere B., Filtjens J., Chasson L., Laprie C., Vivier E, & Ugolini S. (2018). Endogenous glucocorticoids control host resistance to viral infection through the tissue-specific regulation of PD-1 expression on NK cells. Nature immunology, 19(9), 954-962.
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5

Lipopolysaccharide-Induced Endotoxin Tolerance

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LPS (from Escherichia coli strain 055:B5; Sigma) diluted in PBS was injected intraperitoneally (20 µg per gram mouse body weight), and in endotoxin tolerance experiments, 5 µg LPS was injected, in a total volume of 30 µl, into the footpads of mice under anesthesia with 3.5% isoflurane. All LPS injections were performed between 9 a.m. and 10 a.m., and mice were checked every 12 h for signs of distress in survival experiments. Cytokine neutralization was achieved by the intraperitoneal injection of 500 µg anti–IFN-γ (XMG1.2), anti–IL-10 (JES5-2A5), or rat IgG1 (HRPN; all from BioXCell) at the time of LPS priming.
Quatrini L., Wieduwild E., Guia S., Bernat C., Glaichenhaus N., Vivier E, & Ugolini S. (2017). Host resistance to endotoxic shock requires the neuroendocrine regulation of group 1 innate lymphoid cells. The Journal of Experimental Medicine, 214(12), 3531-3541.
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6

MCMV Infection and Immunomodulation Protocol

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Stocks of Smith strain MCMV were generated by homogenizing salivary glands harvested from six-week-old BALB/c mice infected with 2.5 x 103 PFU of MCMV at the age of three weeks. Mice were infected at 2 p.m., by the ip injection of 103 PFU/g or 3 x 103 PFU/g (LD50) MCMV diluted in DMEM. “Non-infected” (NI) mice received DMEM only. For PD-1 blockade experiments, 250 μg anti-PD-1 Ab (clone J43 or RMP1-14) or the appropriate isotype control Ab (Armenian Hamster IgG or IgG2a mAb clone 2A3) (all from BioXCell) were injected i.p. into mice on day 1 PI. For IFN-γ neutralization, 500 μg anti-IFNγ Ab (clone XMG1.2) or Rat IgG1 (HRPN) (both from BioXCell) were injected i.p. into mice on day 1 PI. Spleens and livers were harvested after perfusion at different time points, and were processed for FACS or histology analysis, or weighed and homogenized for RNA or protein extraction. Organs were homogenized in a FastPrep-24™ 5G homogenizer (MP Biomedicals).
Quatrini L., Wieduwild E., Escaliere B., Filtjens J., Chasson L., Laprie C., Vivier E, & Ugolini S. (2018). Endogenous glucocorticoids control host resistance to viral infection through the tissue-specific regulation of PD-1 expression on NK cells. Nature immunology, 19(9), 954-962.
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