HPLC-grade water, HPLC-grade acetonitrile (ACN), and HPLC-grade methanol (MeOH) were obtained from FISHER (USA). Hydrochloric acid (HCl) and sodium chloride (NaCl) were purchased from DUKSAN (Korea). Urea and dithiothreitol (DTT) were purchased from AMRESCO (USA). Phenylmethanesulfonyl fluoride (PMSF), sodium dodecyl sulfate (SDS), and Tris were obtained from USB (USA). Complete protease inhibitor cocktail tablets were acquired from ROCHE (USA), and sequencing-grade modified trypsin was purchased from PROMEGA (USA). Sulfo-NHS acetate and NHS-Activated agarose slurry were obtained from Pierce (USA). All other reagents—iodoacetamide, α-cyano-4-hydroxycinnamic acid (CHCA), and trifluoroacetic acid (TFA)—were purchased from Sigma-Aldrich (USA).
Sodium chloride (nacl)
Manufactured by Duksan Pure Chemicals
Sourced in Cameroon
Sodium chloride is a laboratory chemical that serves as a common salt. It is a white, crystalline solid with a distinct salty taste. Sodium chloride is a simple inorganic compound composed of sodium and chlorine atoms. Its primary function is to provide a source of sodium and chloride ions for various laboratory applications and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Duksan Pure Chemicals (3 mentions)
Dithiothreitol (dtt), by Avantor (2 mentions)
Iodoacetamide, by Merck Group (2 mentions)
Urea, by Avantor (2 mentions)
Sequencing grade modified trypsin, by Promega (2 mentions)
Hplc grade acetonitrile, by Duksan Pure Chemicals (2 mentions)
Hplc grade methanol, by Duksan Pure Chemicals (2 mentions)
Hplc grade water, by Duksan Pure Chemicals (2 mentions)
Ethyl alcohol, by Duksan Pure Chemicals (2 mentions)
Tryptone, by BD (2 mentions)
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Hplc grade water, by Thermo Fisher Scientific (1 mentions)
Complete protease inhibitor cocktail tablet, by Roche (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid chca, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
Proteose peptone, by Merck Group (1 mentions)
Lysozyme, by Fujifilm (1 mentions)
Potassium chloride (kcl), by Duksan Pure Chemicals (1 mentions)
12 protocols using sodium chloride (nacl)
1
Comprehensive HPLC-Mass Spectrometry Protocol
2
Effect of Fat on Listeria monocytogenes Gastric Resistance
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To evaluate the effect of fat on L. monocytogenes resistance to gastric fluid, the simulated gastric fluid (Czuprynski et al., 2002 (link)) was prepared by combining 8.30 g proteose- peptone (Sigma-Aldrich, USA), 3.50 g D-glucose (Samchun Pure Chemical Co. Ltd., Korea), 2.05 g sodium chloride (Duksan Pure Chemicals, Korea), 0.60 g potassium phosphate (Duksan Pure Chemicals), 0.11 g calcium chloride (Samchun Pure Chemical), 0.37 g potassium chloride (Duksan Pure Chemicals), 0.10 g lysozyme (Wako Pure Chemical Industries Ltd., Japan), 50 mg bile salt (Sigma-Aldrich), and 13.30 mg pepsin (Yakuri Pure Chemical Co. Ltd., Japan) per liter of distilled water. The simulated gastric fluid was adjusted to pH 2.0, using 1 N HCl. The frankfurters were transferred from vacuum packages to a filter bag (BagFilter®, Interscience, France) containing 50 mL of simulated gastric fluid, and the samples were homogenized with a pummeler (BagMixer®, Interscience, France) for 30 s. The homogenates were then placed in a water bath at 37℃, and samples were analyzed at 0, 30, 60, 90, and 120 min. The homogenates were diluted with BPW, and 0.1-mL portions of the diluents were then plated on TSAYE and Palcam agar to determine survivals of total bacteria and L. monocytogenes, respectively. The plates were incubated at 30℃ for 48 h, and colonies were manually counted.
3
Glycoproteomic Sample Preparation Protocol
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HPLC-grade acetonitrile (ACN), HPLC-grade water, HPLC-grade methanol, hydrochloric acid (HCl), and sodium chloride (NaCl) were obtained from DUKSAN (Gyungkido, Korea). The BCA protein assay kit was purchased from Pierce (Hercules, CA), and Complete Protease Inhibitor Cocktail Mini Tablets were purchased from Roche (Mannheim, Germany). Dithiothreitol (DTT) and urea were purchased from AMRESCO (Solon, OH). PMSF, Sodium dodecyl sulfate (SDS) and Tris were purchased from USB (Cleveland, OH). Sequencing-grade modified trypsin and LysC were purchased from Promega Corporation (Madison, WI) and Wako (Osaka, Japan), respectively.
All other reagents—concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinus communis agglutinin 120 (RCA120), 2-mercaptoethanol, ammonium bicarbonate (NH4HCO3), sucrose, EDTA, formic acid, iodoacetamide (IAA), trifluoroacetic acid (TFA), PNGase F, and stable isotope-labeled water (H2 18O, 99% atom% 18O)—were purchased from Sigma-Aldrich (St. Louis, MO).
All other reagents—concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinus communis agglutinin 120 (RCA120), 2-mercaptoethanol, ammonium bicarbonate (NH4HCO3), sucrose, EDTA, formic acid, iodoacetamide (IAA), trifluoroacetic acid (TFA), PNGase F, and stable isotope-labeled water (H2 18O, 99% atom% 18O)—were purchased from Sigma-Aldrich (St. Louis, MO).
4
Synthesis of Inorganic Compounds
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Zinc sulfate heptahydrate (ZnSO4·7H2O,
99%, MW 287.56 g mol–1), potassium sulfate (K2SO4, 99%, MW 174.24 g mol–1),
sodium chloride (NaCl, 99%, MW 58.44 g mol–1), anhydrous
magnesium sulfate (MgSO4, 99%, MW 120.37 g mol–1), and ethyl alcohol (C2H5OH, 94%) were purchased
from Duksan Pure Chemicals (South Korea). N,N-Dimethylformamide (C3H7NO, 99.5%,
MW 73.1 g mol–1), oxalic acid dihydrate (H2C2O4·2H2O, 99.5–100.2%,
MW 126.07 g mol–1), 5 N sodium hydroxide standard
solution, potassium permanganate (KMnO4, 99.3%, MW 158.03
g mol–1), and manganese(II) sulfate pentahydrate
(MnSO4·5H2O, 98%, MW 241.08 g mol–1) were purchased from Daejung Chemicals & Metals (South Korea).
Calcium sulfate dihydrate (CaSO4·2H2O,
95%, MW 172.17 g mol–1) was purchased from Oriental
Chemical Industries (South Korea). Strontium nitrate (Sr(NO3)2, 99%, MW 211.63 g mol–1) was purchased
from Sigma-Aldrich (USA) and used to prepare simulant solutions. All
solutions were prepared with deionized (D.I.) water (EXL 5 A16, EXL
Service, USA).
99%, MW 287.56 g mol–1), potassium sulfate (K2SO4, 99%, MW 174.24 g mol–1),
sodium chloride (NaCl, 99%, MW 58.44 g mol–1), anhydrous
magnesium sulfate (MgSO4, 99%, MW 120.37 g mol–1), and ethyl alcohol (C2H5OH, 94%) were purchased
from Duksan Pure Chemicals (South Korea). N,N-Dimethylformamide (C3H7NO, 99.5%,
MW 73.1 g mol–1), oxalic acid dihydrate (H2C2O4·2H2O, 99.5–100.2%,
MW 126.07 g mol–1), 5 N sodium hydroxide standard
solution, potassium permanganate (KMnO4, 99.3%, MW 158.03
g mol–1), and manganese(II) sulfate pentahydrate
(MnSO4·5H2O, 98%, MW 241.08 g mol–1) were purchased from Daejung Chemicals & Metals (South Korea).
Calcium sulfate dihydrate (CaSO4·2H2O,
95%, MW 172.17 g mol–1) was purchased from Oriental
Chemical Industries (South Korea). Strontium nitrate (Sr(NO3)2, 99%, MW 211.63 g mol–1) was purchased
from Sigma-Aldrich (USA) and used to prepare simulant solutions. All
solutions were prepared with deionized (D.I.) water (EXL 5 A16, EXL
Service, USA).
5
Curcumin Nanoformulation Development and Characterization
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Curcumin was purchased from Acros Organics, USA, M.W≈368.38g/mol. PEG 6000, white soft paraffin (M.W≈348g/mol), Triton×®100(M.W≈625g/mol), sodium pyruvate, L-glutamine and 10% fetal bovine serum (FBS) were purchased from Sigma Aldrich, Hamburg, Germany. Liquid white soft paraffin, mineral oil (M.W less than 500g/mol), cremophore EL (M.W≈136.15g/mol), acetone (M.W≈58.08g/mol), potassium dihydrogen phosphate (M.W≈136.086g/ml), sodium hydroxide (M.W≈39.997 g/mol) and Muller Hinton (MH) agar were purchased from Merck Darmstadt, Germany. Sodium chloride (M.W≈58.44g/mol), sodium bicarbonate (NaHCO3, MH≈84.007g/mol) were purchased from Duksan Pure Chemicals, Gyunggido, Korea. Calcium dichloride dehydrate (CaCl2-2H20 M.W≈147.0g/mol), Hank’s balanced salt solution (HBBS) and Dulbecco’s Modified Eagle medium (DMEM) were purchased from Atlanta biologicals, Norcross, Georgia. All chemicals used in study were of analytical grade.
6
Microbiological Analysis of Food and Beverages
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All chemicals used were of analytical grade. Potassium chromate, 0.1 N Sodium hydroxide, silver nitrate, methyl alcohol, ethyl alcohol, and sodium chloride were obtained from Duksan Pure Chemicals (Ansan, Gyeonggi-do, Korea). Sodium hydroxide, sodium hydrogen carbonate, and ammonium hydroxide were purchased from Junsei Chemicals (Tokyo, Japan). Formalin solution, standard methanol, ethanol, pentanol, propanol, and butanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Plate count agar, nutrient agar, potato dextrose agar (PDA), and potato dextrose broth were purchased from Difco (Becton, Dickinson and Company, Sparks, MD, USA). Mannitol egg yolk polymyxin agar (MYP), egg yolk emulsion, and polymyxin B supplement were purchased from Oxoid LTD (Basingstoke, Hampshire, UK). 3M Yeast and Mold Petrifilm was purchased from 3M Health Care (St. Paul, MN, USA). API 50CHB and API 20E were obtained from bioMerieux (Marcy I’Etoile, France).
7
DNA Primer and Protein Plasmid Protocol
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DNA primers and oligonucleotides were purchased from COSMOGENETH (Seoul, Korea). The fluorescent protein plasmid for mScarlet was purchased from Addgene (Watertown, MA). Neutravidin was purchased from ThermoFischer Scientific (Waltham, MA). Enzymes and λ DNA (48.5 kb) were purchased from New England Biolabs (Ipswich, MA). Tryptone and Luria-Bertani broth (LB) were purchased from Becton, Dickinson and Company (Franklin Lakes, NJ). Low melting point (LMP) agarose was purchased from Invitrogen (Carlsbad, CA), and agarose was from Young Sciences, Inc. (Bucheon, Korea). Epoxy was from Permatex (Solon, OH). N-[3-(Trimethoxysilyl)propyl]ethylenediamine was purchased from Acros organics (Fair Town, NJ). Ni-NTA agarose resin and disposable column (empty gravity column) were purchased from Qiagen (Hilden, Germany). Biotin-PEG-succinimidyl carbonate and PEG-succinimidyl valerate were purchased from Laysan Bio Inc (Arab, AL). Sodium chloride, EDTA, and glacial acetic acid were purchased from Duksan (Ansan, Korea). H2O2, H2SO4 and 99.8% ethanol were purchased from JIN Chemical (Hwaseong, Korea). n-Dodecyl-β-d -maltopyranoside (DDM) were purchased from Goldbio (St. Louis, MO). N-Octyl-β-maltopyranoside (OM) were purchased from Anatrace (Maumee, OH). Glutaraldehyde, sodium bicarbonate, thiamine HCl, and other chemicals were purchased from Merck (Darmstadt, Germany).
8
Antioxidant Potential of Xanthine Oxidase Inhibitors
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Xanthine, XO from microbial, allopurinol, aluminum chloride, Folin-Ciocalteau reagent, quercetin, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and PO were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was purchased from Daejung Chemicals & Materials CO., Ltd. (Siheung, Korea). Sodium chloride was purchased from Duksan (Ansan, Korea).
9
Analytical Reagents for Protein Analysis
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HPLC-grade acetonitrile (ACN), HPLC-grade water, HPLC-grade methanol (MeOH), hydrochloric acid (HCl), and sodium chloride (NaCl) were obtained from DUKSAN (Kyungkido, Korea). Acetic acid was purchased from TEDIA (Fairfield, OH, USA). Brilliant Blue G-250 and a Bio-Rad protein assay kit were purchased from Bio-Rad (Hercules, CA, USA), and Complete Protease inhibitor cocktail was purchased from Roche (Mannheim, Germany). Other reagents, including 2-mercaptoethanol (β-ME), ammonium bicarbonate (ABC), ammonium persulfate (APS), EDTA, iodoacetamide (IAA), magnesium chloride, MES hydrate, potassium chloride, sodium bisulfite, sodium deoxycholate (SDC), sodium pyrophosphate, and trifluoroAcetic acid (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
10
Aflatoxin Extraction and Derivatization
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Twenty-five grams of the sample was added to 2.5 g of sodium chloride (Duksan Pure Chemicals Co. Ltd., Korea) and extracted with 100 mL of acetonitrile:water (86:14, v/v) (J.T. Baker., USA) by blending at high speed for 5 minutes and then filtered through Advantec No. 1 filter paper (Whatman TM Maidtone, UK). Five ml of the filtrate sample was diluted with 20 mL of distilled water and passed through an immunoaffinity column (IAC) (Vicam Nixa, USA). AFT was eluted from the column by applying 1.5 mL of methanol and the extract was concentrated under nitrogen in a water bath at 50°C. Then, 200 μL of trifluiacetic acid (Sigma-Aldrich, USA) and 800 μL of mobile phase (20% acetonitrile) were added and then derivatized for 20 minutes. The derivatized solution was filtered through a 0.45 μm filter (Milipore, USA) and the filtrate was analyzed.
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