Cells were harvested from spleen, mesenteric lymph nodes, bone marrow or thymus. Tissues were crushed into PBS through a 40 micron cell strainer using the back of a 1 mL syringe plunger. Cell preparations were subjected to hypotonic lysis to remove erythrocytes, stained and analyzed using a Fortessa (BD). CD1d(PBS57) tetramer was obtained from the NIH Tetramer Core Facility. Flow cytometry antibodies used in this study were purchased from Biolegend (anti-B220-PacificBlue [clone RA3-6B2], anti-CD107a-Fitc [1D4B], anti-CD11b-AlexaFluorophore 488 [M1/70], anti-CD11c-APC [N418], anti-CD11c-PE/Cy7 [N418], anti-CD25-AlexaFluorophore 488 [PC61], anti-CD44-Bv421 [IM7], anti-CD44-PE/Cy7 [IM7], anti-CD45-Bv711 [30-F11], anti-CD45.1-Bv711 [A20], anti-CD4-APC [RM4-5], anti-CD4-PacificBlue [RM4-5], anti-CD69-PE [H1.2F3], anti-CD8-Bv650 [53-6.7], anti-FoxP3-PE [MF-14], anti-Gr1-PE [RB6-8C5], anti-Gr1-PE/Cy7 [RB6-8C5], anti-IFNγ-Bv421 [XMG1.2], anti-LAG3-Pe [C9B7W], anti-MHC I-A/I-E-Bv510 [M5/114.15.2], anti-NK1.1-FITC [PK136], anti-NK1.1-PE/Cy7 [PK136], anti-PD-1-PE/Cy7 [29F.1A12], Tim3-APC [RMT3-23]) and Affymetrix (anti-CD19-PE [MB19-1], anti-GrzB-Pe/Cy7 [NGZB]).
Anti cd44 bv421
Manufactured by BioLegend
Sourced in United States
Anti-CD44 BV421 is a fluorochrome-conjugated antibody that binds to the CD44 cell surface antigen. CD44 is a glycoprotein involved in cell-cell interactions, cell adhesion and migration. The BV421 fluorochrome has an excitation maximum of 405 nm and an emission maximum of 421 nm.
Automatically generated - may contain errors
Lab products found in correlation
Facsmelody, by BD (2 mentions)
Anti cd24, by BioLegend (2 mentions)
Anti e cadherin bb700, by BD (2 mentions)
Anti cd11c apc, by BioLegend (1 mentions)
Anti cd4 apc, by BioLegend (1 mentions)
Anti cd44 pe cy7, by BioLegend (1 mentions)
Anti cd4 pacific blue, by BioLegend (1 mentions)
Anti cd45 bv711, by BioLegend (1 mentions)
Anti cd11c pe cy7, by BioLegend (1 mentions)
Fortessa, by BD (1 mentions)
Anti gr1 pe cy7, by BioLegend (1 mentions)
Anti nk1.1 fitc, by BioLegend (1 mentions)
Anti pd 1 pe cy7, by BioLegend (1 mentions)
Anti nk1.1 pe cy7, by BioLegend (1 mentions)
3 protocols using anti cd44 bv421
1
Multiparameter Analysis of Immune Cell Subsets
2
E-cadherin, CD44, and CD24 Analysis
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At the conclusion of the TGFβ time course, cells were counted and resuspended in 500 µL 1% FBS (Cytiva, Marlborough, MA, USA) in PBS with anti-E-cadherin BB700 (BD Biosciences, Franklin Lakes, NJ, USA; #745965, 1:100), anti-CD44 BV421 (BioLegend, San Diego, CA, USA; #562890, 1:100), and anti-CD24 (BioLegend, San Diego, CA, USA; #311104, 1:100), and incubated on ice for 90 min. Following incubation, cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelodyTM (BD Biosciences, Franklin Lakes, NJ, USA).
3
Flow Cytometry Analysis of EMT Markers
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At the conclusion of the TGFβ time course, cells were counted and resuspended in 500 µL 1% FBS (Cytiva) in PBS with anti-E-cadherin BB700 (BD Biosciences #745965, 1:100), anti-CD44 BV421 (BioLegend #562890, 1:100), and anti-CD24 (BioLegend #311104, 1:100) and incubated on ice for 90 minutes. Following incubation, cells were pelleted and washed twice with 1% FBS in PBS and subjected to flow cytometry using a FACSMelody TM (BD Biosciences).
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