Polyclonal anti mapk and anti pmapk antibodies

Water-soluble progesterone (PG), anesthetic MS-222 and apocynin were obtained from Sigma (St. Louis, MO, USA). Collagenase (280 U/mg) and catalase (11,000 U/mg) were purchased from Wako (Osaka, Japan), hCG was from Teikoku Zoki (Tokyo, Japan). Fluorogenic caspase-3 substrate IV was purchased from Calbiochem (La Jolla, CA, USA). Hydrogen peroxide colorimetric/fluorometric assay kit was from BioVision (Milpitas, CA, USA). Polyclonal anti-MAPK and anti-pMAPK antibodies were from Cell Signaling (Beverly, MA, USA), biotinylated anti-rabbit IgG was from Vector Laboratories (Burlingame, CA, USA). The Streptavidin Biotin Complex Peroxidase Kit, protein assay CBB solution, SOD (5000 U/mg) and BHA were from Nacalai Tesque (Kyoto, Japan). 2′,7′-dichlorofluorescein diacetate (DCFDA) Cellular ROS Detection Assay Kit was obtained from Abcam (Cambridge, UK), EUK 134 and MITO-TEMPO were ordered from Santa Cruz (Santa Cruz Biotechnology, Dallas, TX, USA). Other chemicals were obtained from Wako and Nacalai Tesque.

Anesthetic MS-222 and water-soluble PG were purchased from Sigma (St. Louis, MO, USA). Collagenase (280 U/mg) was obtained from Wako (Osaka, Japan) and hCG was from Teikoku Zoki (Tokyo Japan). Polyclonal anti-MAPK and anti-pMAPK antibodies were from Cell Signaling (Beverly, MA), biotinylated anti-rabbit IgG was from Vector Laboratories (Burlingame, CA, USA), and the Streptavidin Biotin Complex Peroxidase Kit was from Nacalai Tesque (Kyoto, Japan). Polyvinulidene difluoride (PVDF) membranes Immobilon were purchased from Merck Millipore (Carrigtwohill, Ireland). Protein assay CBB solution was from Nacalai Tesque (Kyoto, Japan). Specific MEK inhibitor U0126 was from Promega (Madison, WI) and MMP inhibitor GM6001 was from Calbiochem (Darmstadt, Germany). Other chemicals were obtained from Wako and Nacalai Tesque.

Water-soluble progesterone (PG), anesthetic MS-222, and an ATP Bioluminescence Assay Kit CLS II were purchased from Sigma (St. Louis, MO, USA). Human chorionic gonadotropin (hCG) was obtained from Teikoku Zoki (Tokyo, Japan), and collagenase (280 U/mg) was obtained from Wako (Osaka, Japan). Fluorogenic caspase-3 substrate IV was purchased from Calbiochem (La Jolla, CA, USA), and Apo-ONE homogenous caspase 3/7 assay was obtained from Promega (Madison, WI, USA). Polyclonal anti-cyclin B2 antibody was ordered from Santa Cruz (Dallas, TX, USA), and biotinylated anti-rabbit IgG was purchased from Vector Laboratories (Burlingame, CA, USA). Polyclonal anti-MAPK and anti-pMAPK antibodies were purchased from Cell Signaling (Beverly, MA, USA). The Streptavidin Biotin Complex Peroxidase Kit and CBB protein assay kit were obtained from Nacalai Tesque (Kyoto, Japan), and the bioluminescent ApoSENSOR ADP/ATP ratio assay kit was purchased from BioVision (Mountain View, CA, USA). Other chemicals were obtained from Wako and Nacalai Tesque.