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Cytochrome c cyt

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Cytochrome c (CYT) is a protein found in the mitochondria of eukaryotic cells. It functions as an electron carrier in the electron transport chain, which is a crucial step in the process of cellular respiration and ATP production.

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Lab products found in correlation

Ammonium nitrate, by Thermo Fisher Scientific (1 mentions) Thioglycolic acid, by Merck Group (1 mentions) Cadmium acetate dihydrate, by Merck Group (1 mentions) Sodium ascorbate, by Merck Group (1 mentions) Magnesium sulfate, by Merck Group (1 mentions) Sodium hydroxide (naoh), by ITW Reagents (1 mentions) P toluenesulfonyl chloride, by Merck Group (1 mentions) 3 aminopropyltriethoxysilane, by Merck Group (1 mentions) Bioultra 8000, by Merck Group (1 mentions) Sodium tellurite, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Tetraethyl orthosilicate, by Merck Group (1 mentions) Chloroform, by Carlo Erba (1 mentions) Monobromobimane, by Merck Group (1 mentions) Cumene hydroperoxide, by Merck Group (1 mentions) Erastin, by Merck Group (1 mentions) Liproxstatin 1, by Merck Group (1 mentions) Disodium sulfide, by Merck Group (1 mentions) Diallyl tetrasulfide, by Thermo Fisher Scientific (1 mentions) Glutathione reductase, by Merck Group (1 mentions)

2 protocols using cytochrome c cyt

1

Synthesis and Characterization of Nanoparticles

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Thioglycolic acid (TGA, C2H4O2S, ≥99%), cadmium acetate dihydrate (Cd(CH3COO)2·2H2O, 99.5%), (3-aminopropyl)triethoxysilane (APTES, H2N(CH2)3Si(OC2H5)3, 99%), tetraethyl orthosilicate (TEOS, SiC8H20O4, 98%), ammonia (NH3, ≥99.9%), cetyltrimethylammonium bromide (CTAB, C19H42BrN, ≥98%), poly(ethylene glycol) BioUltra 3000, poly(ethylene glycol) BioUltra 6000, poly(ethylene glycol) BioUltra 8000, p-toluenesulfonyl chloride (CH3C6H4SO2Cl, ≥98%), hydrochloric acid (HCl, 37%), magnesium sulfate (MgSO4, ≥99.5%), sodium ascorbate, bovine serum albumin (BSA), lysozyme (LYS), carbonic anhydrase (CA), ovalbumin (OVA), hemoglobin (Hb), myoglobin (Myb), and cytochrome c (CYT) were purchased from Sigma-Aldrich (Lisbon, Portugal). Sodium hydroxide (NaOH, ≥98%) was produced by Panreac. Sodium tellurite (Na2TeO3, 99.5%) and ammonium nitrate (NH4NO3, 99.9%) were purchased from Alfa Aesar. Sodium borohydride (NaBH4, 99%), ethylene glycol (C2H6O2, ≥99.5%) and (3-isocyanatopropyl)triethoxysilane (IPTES, (C2H5O)3Si(CH2)3NCO, 95%) were produced by Fluka. Methanol (MeOH), ethanol (EtOH), tetrahydrofuran (THF, C4H8O, ≥99.9%), and chloroform (CHCl3) were purchased by Carlo Erba Reagents. All the reagents and solvents were of analytical reagent grade and were used as received. All cell culture reagents were purchased from Gibco (Alfagene, Lisbon, Portugal) unless stated otherwise.
Marcelo G., Ariana-Machado J., Enea M., Carmo H., Rodríguez-González B., Luis Capelo J., Lodeiro C, & Oliveira E. (2018). Toxicological Evaluation of Luminescent Silica Nanoparticles as New Drug Nanocarriers in Different Cancer Cell Lines. Materials, 11(8), 1310.
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2

Redox Signaling Compound Synthesis and Detection

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3-Aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na2S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7- (Diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3-MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), S- nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-D-1-thiogalactopyranoside (IPTG) were obtained from Sigma. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red was obtained from ThermoFisher. DEPMPO was obtained from Enzo. H2O2 was obtained from Roth. Glutathione (GSH) and 5- aminolevulinic acid hydrochloride was obtained from Merck. Sodium disulfide (Na2S2), sodium tetrasulfide (Na2S4), and 3',6'-Di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS34SSG and GSSSSG was performed as described previously 43 . CSSSC was synthesized as described below.
Barayeu U., Schilling D., Eid M., Xavier da Silva T.N., Schlicker L., Mitreska N., Zapp C., Gräter F., Miller A.K., Kappl R., Schulze A., Friedmann Angeli J.P, & Dick T.P. (2022). Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals. Nature Chemical Biology, 19(1), 28-37.
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