For ferritin and protein determination, the cells were thawed and then centrifuged at 13,000× g for 15 min at 4 °C to discard the cellular debris from the proteins contained in the supernatant. The ferritin content was determined by the Human Ferritin ELISA Kit (Thermo Scientific) and was measured using a microplate reader (infinite 200 pro Tecan i-control, Switzerland) at 450 nm with a reference wavelength set at 550 nm according to the kit instructions. The Coomassie Plus Assay Kit (Thermo Scientific) was used to determine the total protein content at 595 nm by the microplate reader. The determination of the latter was necessary to normalize the ferritin concentration.
Human ferritin elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Human Ferritin ELISA Kit is a quantitative in vitro diagnostic test used to measure the concentration of ferritin in human serum or plasma samples. Ferritin is an iron-storage protein that can be used as a biomarker for various medical conditions. This kit employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify ferritin levels.
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Lab products found in correlation
Advia chemistry 2400 xpt, by Siemens (2 mentions)
Human troponin elisa kit, by Thermo Fisher Scientific (2 mentions)
Xt 1800i, by Sysmex (2 mentions)
Coomassie plus assay kit, by Thermo Fisher Scientific (1 mentions)
Cellytic m, by Merck Group (1 mentions)
Coomassie plus protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Caco 2 cell line, by Merck Group (1 mentions)
4 protocols using human ferritin elisa kit
1
Ferritin and Protein Quantification
2
Caco-2 Cell Culture and Characterization
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PBS phosphate-buffered saline (PBS) solution for cell culture pH 7.4 (Gibco, Thermo Fisher Scientific), 200 µL Cellytic M (Sigma C2978), Human Ferritin ELISA Kit (Thermo Scientific), Coomassie Plus™ protein assay reagent (Thermo Scientific), 96-well microplates for protein determination (Greiner bio-one, Frickenhausen, Germany) were all purchased.
The Caco-2 cell line was provided by Sigma at passage 54 and stored in liquid nitrogen.
The Caco-2 cell line was provided by Sigma at passage 54 and stored in liquid nitrogen.
3
Comprehensive Blood Assays in Research
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First, 5 mL of peripheral venous blood in a plane vacutainer tube was withdrawn for the assessment of a complete blood count (CBC) (using Sysmex XT-1800i, Kobe, Japan), liver functions, alanine transaminase (ALT), and aspartate aminotransferase (AST), using spectrophotometric assay on Advia chemistry 2400 XPT, Siemens Healthineers (Erlangen, Germany), automated quantitative C-reactive protein (CRP), and lactate dehydrogenase (LDH) (using Roche Diagnostics, Mannheim, Germany).
Then, 5 mL of peripheral venous blood was obtained in a vacutainer tube containing citrate for the assessment of an automated quantitative D-Dimer using D-Dimer Reagent Specification commercially available by HEALES; Shenzhen Housing Technology; China.
The enzyme-linked immunosorbent assay (ELISA) is an effective method for measuring cytokines. The Human IL-6 ELISA kit tested serum IL-6 (Shanghai Sunred Biological Technology Co., Ltd., Shanghai, China). Serum ferritin and troponin were measured using the Human Ferritin ELISA kit and a Human Troponin ELISA kit (eBioscience, San Diego, CA, USA). The manufacturer’s ELISA steps were followed.
Then, 5 mL of peripheral venous blood was obtained in a vacutainer tube containing citrate for the assessment of an automated quantitative D-Dimer using D-Dimer Reagent Specification commercially available by HEALES; Shenzhen Housing Technology; China.
The enzyme-linked immunosorbent assay (ELISA) is an effective method for measuring cytokines. The Human IL-6 ELISA kit tested serum IL-6 (Shanghai Sunred Biological Technology Co., Ltd., Shanghai, China). Serum ferritin and troponin were measured using the Human Ferritin ELISA kit and a Human Troponin ELISA kit (eBioscience, San Diego, CA, USA). The manufacturer’s ELISA steps were followed.
4
Comprehensive Blood Analysis Protocol
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First, 5 mL of peripheral venous blood in a plane vacutainer tube was withdrawn for the assessment of a complete blood count (CBC) (using Sysmex XT-1800i, Kobe, Japan), liver functions, alanine transaminase (ALT), and aspartate aminotransferase (AST), using a spectrophotometric assay on Advia chemistry 2400 XPT, Siemens Healthineers (Erlangen, Germany), automated quantitative C-reactive protein (CRP), and lactate dehydrogenase (LDH) (using Roche Diagnostics, Mannheim, Germany).
Following this, 5 mL of peripheral venous blood was obtained in a vacutainer tube containing citrate for the assessment of an automated quantitative D-dimer using the D-Dimer Reagent Specification commercially available from HEALES, Shenzhen Housing Technology, China.
The enzyme-linked immunosorbent assay (ELISA) is an effective method for measuring cytokines. The Human IL-6 ELISA kit tested serum IL-6 (Shanghai Sunred Biological Technology Co., Ltd., Shanghai, China). Serum ferritin and troponin were measured using the Human Ferritin ELISA kit and a Human Troponin ELISA kit (eBioscience, San Diego, CA, USA). The manufacturer’s ELISA steps were followed.
Following this, 5 mL of peripheral venous blood was obtained in a vacutainer tube containing citrate for the assessment of an automated quantitative D-dimer using the D-Dimer Reagent Specification commercially available from HEALES, Shenzhen Housing Technology, China.
The enzyme-linked immunosorbent assay (ELISA) is an effective method for measuring cytokines. The Human IL-6 ELISA kit tested serum IL-6 (Shanghai Sunred Biological Technology Co., Ltd., Shanghai, China). Serum ferritin and troponin were measured using the Human Ferritin ELISA kit and a Human Troponin ELISA kit (eBioscience, San Diego, CA, USA). The manufacturer’s ELISA steps were followed.
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