Pe conjugated anti mouse cd40

CH (MW 50–190 KDa), TPP, OVA, and poly I:C were purchased from Sigma-Aldrich (St. Louis, MO, USA). FITC-conjugated anti-mouse IFN-γ, PE-conjugated anti-mouse CD40, and anti-OVA-specific (SIINFEKL/H-2Kb) antibodies and mouse TNF-α, IL-1β, IL-6, and IL-12p70 ELISA Ready-SET-Go kits were purchased from eBioscience (San Diego, CA, USA). A FITC-conjugated anti-mouse CD11c antibody and PE-conjugated anti-mouse CD8a, CD80, CD86, MHC class I, and MHC class II antibodies and a mouse IFN-γ ELISA kit were purchased from Biolegend (San Diego, CA, USA). RPMI 1640 and fetal bovine serum (FBS) were acquired from Biowest (Nuaille, France). Granulocyte-macrophage colony-stimulating factor (GM-CSF) was purchased from JW Creagene (Gyeonggi, South Korea). HPV-16 E7 peptide (MW 2.4 kDa, AGQAEPDRAHYNIVTFCCKCDS)37 was purchased from AnyGen Co. (Seoul, South Korea). All other materials were of analytical grade and used without further purification.