Apoptosis assays were performed with allophycocyanin-conjugated annexin V and 7-aminoactinomycin D (BD Biosciences, San Jose, CA, USA). Cells were analyzed for fluorescence on a Guava easyCyte HT Flow Cytometer (MilliporeSigma, Burlington, MA, USA) or a BD Canto flow cytometer.
Allophycocyanin conjugated annexin 5 and 7 aminoactinomycin d
Manufactured by BD
Sourced in United States
Allophycocyanin-conjugated annexin V and 7-aminoactinomycin D are fluorescent dyes used in flow cytometry and other cell-based assays. Allophycocyanin-conjugated annexin V binds to phosphatidylserine, which is externalized on the surface of apoptotic cells. 7-aminoactinomycin D is a DNA-binding dye that can be used to detect cell death. These reagents are commonly used together to identify and quantify apoptotic cells.
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2 protocols using allophycocyanin conjugated annexin 5 and 7 aminoactinomycin d
1
Apoptosis Assessment by Flow Cytometry
2
Apoptosis Assessment by Flow Cytometry
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Apoptosis assays were performed with allophycocyanin-conjugated annexin V and 7-aminoactinomycin D (BD Biosciences, San Jose, CA, USA). Cells were analyzed for fluorescence on a Guava easyCyte HT Flow Cytometer (MilliporeSigma, Burlington, MA, USA) or a BD Canto flow cytometer.
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