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Recombinant murine wnt3a protein

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Recombinant murine Wnt3a protein is a purified protein derived from a recombinant source. It is a member of the Wnt protein family, which play key roles in cellular signaling pathways.

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Lab products found in correlation

Renca, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions) Hek293t, by Fujifilm (1 mentions) Recombinant human wnt3a protein, by R&D Systems (1 mentions) Rpmi medium, by Fujifilm (1 mentions) Pcr mycoplasma detection kit, by Takara Bio (1 mentions) Hek293t, by American Type Culture Collection (1 mentions)

2 protocols using recombinant murine wnt3a protein

1

Wnt3a Signaling Pathway Evaluation

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Recombinant murine Wnt3a protein (PeproTech, 315-20) was used for Wnt signaling studies (100 ng/mL). For protease protection studies, cells processed after 30 min of Wnt3a treatment at 37 °C. For luciferase assays, Wnt3a was added for 12 h.
Albrecht L.V., Bui M.H, & De Robertis E.M. (2019). Canonical Wnt is inhibited by targeting one-carbon metabolism through methotrexate or methionine deprivation. Proceedings of the National Academy of Sciences of the United States of America, 116(8), 2987-2995.
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2

Cell Culture and Reagent Preparation

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The HEK293T (human embryonic kidney cell), CT26 (murine colon cancer), RENCA (murine renal cell carcinoma), and A20 (murine lymphoma) cell lines were purchased from ATCC (Manassas, VA). The HEK293T and CT26 cell lines were maintained in DMEM (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 10% foetal calf serum (FCS; Cytiva, Tokyo, Japan). The RENCA and A20 cell lines were maintained in RPMI medium (FUJIFILM Wako Pure Chemical Corporation) supplemented with 10% FCS. All tumour cells were confirmed to be Mycoplasma (−) using a PCR Mycoplasma Detection Kit (TaKaRa, Shiga, Japan) according to the manufacturer’s instructions before use. Recombinant human Wnt3a protein was obtained from R&D (Minneapolis, MN). Recombinant murine Wnt3a protein was purchased from PeproTech (Cranbury, NJ). Rat anti-mouse PD-1 mAb (RMP1-14) and control rat IgG2a mAb (RTK2758) were obtained from BioLegend (San Diego, CA).
Ishino T., Kawashima S., Tanji E., Ueno T., Ueda Y., Ogasawara S., Sato K., Mano H., Ishihara S., Kato N., Kawazu M, & Togashi Y. (2023). Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British Journal of Cancer, 128(6), 1166-1175.
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