Dnase 1 kit

Total RNA was extracted from plasma samples from 24 PD patients (Dnr_8 to Dnr_31) and 11 healthy controls (Dnr_35 to Dnr_45) using an exoRNeasy Serum/Plasma Maxi Kit (50) (Qiagen, Redwood City, CA, USA; cat. #77064). The DNA in the RNA residue was digested using a DNase I kit (CW Bio, Cambridge, MA, USA; cat. #CW2090). The RNA was reverse transcribed into complementary DNA (cDNA) using a HiFi‐MMLVcDNA kit (CW Bio; cat #CW0744) according to the manufacturer's instructions. PCR was performed on the cDNA using UltraSYBR Mixture (With ROX; CW Bio; cat #CW0956). No electrophoresis was performed because of a low RNA yield. β‐actin was used as a housekeeping gene. Data were calculated as relative expressions according to the ^ΔΔC(t) principle.18, 19 The primer sequences were 5′‐GCA AGC CTA ACT CAA GCC ATT‐3′ and 5′‐TCA AGC CGA CTC TCC ATA CC‐3′ for GAS5:46; and 5′‐AGG TGG GAG GAT CGC TTG A‐3′ and 5′‐ACC ATA TTG ATG CCG AAC TTA GTG‐3′ for lnc‐MKRN2‐42:1.