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Cell apoptosis histone dna elisa detection kit

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The Cell Apoptosis Histone-DNA ELISA Detection Kit is a quantitative sandwich enzyme immunoassay designed for the detection of histone-complexed DNA fragments, a marker of apoptosis, in cell and tissue lysates.

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Apoptosis detection kit (87 citations) Apoptosis ELISA kit (11 citations) Histone-DNA ELISA Detection Kit (8 citations) Histones (4 citations) Histone DNA apoptosis ELISA kit (3 citations) ELISA detection kit (3 citations) Histone-DNA Apoptosis ELISA Detection Kit (2 citations) ELISAs (2 citations)

3 protocols using cell apoptosis histone dna elisa detection kit

1

Cell Apoptosis Quantification by ELISA

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As described in our previous studies [6 (link),7 (link)], following applied treatment, cell apoptosis was quantified by the Cell Apoptosis Histone DNA ELISA Detection Kit (Roche, Palo Alto, CA), according to the manufacturer's protocol [23 (link)].
Jiang H., Liu W., Zhan S.K., Pan Y.X., Bian L.G., Sun B., Sun Q.F, & Pan S.J. (2016). GSK621 Targets Glioma Cells via Activating AMP-Activated Protein Kinase Signalings. PLoS ONE, 11(8), e0161017.
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2

Cell Apoptosis Quantification by ELISA

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As described in our previous studies [6 , 11 (link)], the Cell Apoptosis Histone-DNA ELISA Detection Kit (Roche, Palo Alto, CA) was applied to quantify cell apoptosis. ELISA OD was recorded as a quantitative indicator of cell apoptosis.
Li J.P., Huang Z.J., Lu X.S., Zhou Y.C., Shao Y., He X.P., Chen S.R., Wang D.D., Qin L.S, & Sun W.H. (2016). Pre-clinical characterization of PKC412, a multi-kinase inhibitor, against colorectal cancer cells. Oncotarget, 7(47), 77815-77824.
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3

Quantifying Cell Apoptosis via ELISA

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The Cell Apoptosis Histone-DNA ELISA Detection Kit (Roche, Palo Alto, CA) was used to quantify HGC-27 cell apoptosis with indicated treatment/s according to the manufacturer's protocol. Briefly, the cytoplasmic histone/DNA fragments from cells after treatments were extracted and bound to immobilized anti-histone antibody. Subsequently, the peroxidase-conjugated anti-DNA antibody was then added for the detection of immobilized histone/DNA fragments. After addition of substrate for peroxidase, the spectrophotometric absorbance of the samples was determined using a plate reader at a test wavelength of 405 nm.
Ji D., Zhang Z., Cheng L., Chang J., Wang S., Zheng B., Zheng R., Sun Z., Wang C., Zhang Z., Liu R., Zhang X., Liu X., Wang X, & Li J. (2014). The Combination of RAD001 and MK-2206 Exerts Synergistic Cytotoxic Effects against PTEN Mutant Gastric Cancer Cells: Involvement of MAPK-Dependent Autophagic, but Not Apoptotic Cell Death Pathway. PLoS ONE, 9(1), e85116.
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