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10 20 system

Manufactured by Brain Products
Sourced in Germany

The 10-20 system is a standardized method for the placement of electrodes on the scalp for electroencephalography (EEG) recording. It is a widely used technique for the identification of specific brain regions and their corresponding electrode locations. The system ensures consistent and reproducible electrode placement, allowing for accurate data collection and comparison across different studies or clinical applications.

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10 protocols using 10 20 system

1

EEG Protocol for Visual Perception

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Electroencephalographic data were recorded from a 64-electrode scalp cap using the 10–20 system (Brain Products, Munich, Germany) with reference electrodes on the left and right mastoids. The vertical electrooculogram (EOG) was recorded with placed above and below the left eye. EEG and EOG activity was amplified at 0.01–100 Hz band-pass and sampled at 500 Hz. All of the electrode impedances were maintained below 5 kΩ.
The EEG data were pre-processed and analyzed using Matlab R2011b software (MathWorks) and the EEGLAB toolbox (Delorme and Makeig, 2004 (link)). The EEG data for each electrode were down-sampled to 250 Hz and re-referenced to the grand averages. The signal was then passed through a 0.01- to 30-Hz band-pass filter. Time windows of 200 ms before and 700 ms after the onset of the picture were segmented. EOG artifacts were corrected using an independent component analysis (ICA) (Jung et al., 2001 (link)) (Supplementary Figure S1). Epochs with amplitudes that exceeded ±50 μV at any electrode were excluded from the average (5.6 ± 0.6% trials were excluded).
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2

EEG Recording of Visual Attention

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Subjects seated in a comfortable chair in a silent room which temperature was maintained between 24 and 26 °C. They were instructed to focus on the stimuli, keep their eyes open, and gaze at a fixation point on the screen. A curtain was used to block the subjects’ view of their forearms. EEG data were collected using 64 Ag—AgCl scalp electrodes placed according to the International 10–20 system (Brain Products GmbH; pass band: 0.01–100 Hz; sampling rate: 1000 Hz). The nose was used as reference, and electrode impedances were kept lower than 10 kΩ. Electrooculographic signals were simultaneously recorded using two surface electrodes, one placed ∼10 mm below the left eye and the other placed ∼10 mm from the outer canthus of the left eye.
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3

EEG Recording and Analysis Protocol

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Twenty-one undergraduate and graduate students were recruited to participate as paid volunteers in the collection of the actual dataset. Nine were females and twelve were males (mean age: 20.95 years old). All the subjects were right-handed, with normal or corrected to normal visual acuity, and they did not know or see the experimental paradigm before the experiment. The details of the experiment materials and the paradigm can be found in this research [18 (link)]. EEG recordings at 64 locations were collected according to the standard 10-20 system (Brain Products GmbH, Gilching, Germany). The EEG data were referenced online against the left and right mastoids. Meanwhile, we also collected the vertical and horizontal electrooculogram (EOG) from four electrodes which were placed above and below the right eye and on the outer canthus of the right and left eyes, respectively. All impedances were less than 10 kΩ for each electrode. The EEG and EOG for each participant were recorded with a 500 Hz sampling rate, and the data were filtered between 0.01 and 100 Hz using a band-pass filter. The signals from six electrodes (i.e., “HEOL,” “VEOD,” “HEOR,” “VEOU,” “M1,” and “M2”) were not involved in further analysis.
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4

EEG Data Preprocessing and Analysis Protocol

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Electroencephalography (EEG) data were recorded from a 63-electrodes scalp cap using the 10–20 system (Brain Products, Munich, Germany). The channel TP10 was used as the reference during recording. Two electrodes located above and below the left eye were used to measure the electrooculogram (EOG). EEG and EOG activities were amplified at 0.01–100 Hz band-passes and sampled at 500 Hz. All electrode impedances were maintained below 5 kΩ.
Electroencephalography (EEG) data were preprocessed and analyzed using MATLAB R2011b (MathWorks) and EEGLAB toolbox (Delorme and Makeig, 2004 (link)). EEG data at each electrode were re-referenced to the average of the left and right mastoids before further analysis. Then the signal passed with 0.01–30 Hz band-pass filter. Time windows of 200 ms before and 1000 ms after the onset of picture stimuli were segmented from EEG and the whole epoch was baseline-corrected by the 200 ms prior to the onset of the picture. EOG artifacts were corrected using an independent component analysis (ICA) (Jung et al., 2001 (link)). Epochs with amplitude values exceeding ± 50 μV at any electrode were excluded from the average, and all trials with incorrect responses were excluded from further analysis [Rejected epochs: 16.75 ± 6.04 (HCs); 18.25 ± 2.35 (IADs)].
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5

EEG Data Acquisition and Analysis Protocol

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The EEG was recorded from a 32-channel scalp standard cap using the 10/20 system (Brain Products, Munich, Germany). We monitored the vertical electrooculogram (VEOG) by placing electrodes 1 cm from the outer canthi of the right eye and the horizontal electrooculogram (HEOG) by placing electrodes above and below the left eye. All electrode recordings were referenced online to FCz, and inter-electrode impedances were maintained below 5kΩ. The EEG and EOG signals were amplified using a 0.01–100 Hz band-pass filter and continuously sampled at 500 Hz/channel for offline analysis.
After data acquisition, EEG data were imported into the open-source MATLAB toolboxes EEGLAB and Letswave for neurophysiological data analysis. The EEG recordings were re-referenced to the average of the two mastoids and band-pass filtered between 0.1 and 30 Hz. Independent component analysis (ICA) was used to isolate ocular (blink and saccade) and other remaining artifacts. Epochs were extracted from 200 ms prior to the stimulus onset to 1000 ms post-stimulus interval, and baseline correction was performed using the mean voltage in the 200 ms interval preceding stimulus onset. ERPs were computed offline by averaging according to the experimental design. The data analyses were conducted using MATLAB R2013b (MathWorks, Natick, USA) and SPSS Statistics 20.0 (IBM, Somers, USA).
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6

Resting-State EEG Recording Protocol

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During the EEG recording, participants were seated in an isolated sound-shielded room, connected to a recording room via a one-way glass window, and asked to rest comfortably with eyes closed. EEG signals were collected using a 64-channel amplifier (based on the 10–20 system; Brain Products, Gilching, Germany) with a sample rate of 500 Hz, with the reference set at FCz, and the ground located between FPz and Fz. The vertical and horizontal electrooculogram (VEOG and HEOG) were recorded on the right and left eye respectively, and scalp impedance were kept below 5 kΩ. The EEG and EOG activities were amplified with a DC 0.1–1000 Hz band pass, and the recordings lasted for 5 min.
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7

Evaluating Cognitive Tasks via EEG

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EEG data were collected during the Pain Judgment and Hands Counting Tasks. Participants sat on a comfortable chair in a silent and temperature‐controlled room. They were instructed to focus on the stimuli, keep their eyes open, and gaze at a fixation point on the screen. EEG data were recorded using 64 Ag‐AgCl scalp electrodes placed according to the International 10–20 system (Brain Products GmbH; bandpass filter: 0.01–100 Hz; sampling rate: 1000 Hz). The electrode‐to‐skin impedances were kept below 10 kΩ for all electrodes. Electro‐oculographic (EOG) signals were simultaneously recorded using surface electrodes to monitor ocular movements and eye blinks.
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8

EEG Recording with 32-Channel Setup

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The EEG was recorded from 32 channels using the standard 10–20 system (Brain Products, Gilching, Germany) with a bandpass from 0.01 to 100 Hz and a 500 Hz sampling rate. All channels were online referenced to FCz during recording. Recording impedance for all electrodes was held beneath 10 kΩ.
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9

EEG Analysis of Facial Stimuli

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Electroencephalographic data were recorded from a 64-electrode scalp cap using the 10–20 system (Brain Products, Munich, Germany) with the reference on the left and right mastoids. A vertical electrooculogram (EOG) was recorded with electrodes placed above and below the left eye. EEG and EOG data were amplified, band-pass filtered (0.01–100 Hz), and sampled at 500 Hz. All electrode impedances were maintained below 5 kΩ.
EEG data were pre-processed and analyzed using MATLAB R2011b (Math Works, US) and EEGLAB toolbox (Delorme and Makeig, 2004 (link)). EEG data at each electrode were down-sampled to 250 Hz, re-referenced to the grand average, and band-pass filtered (0.01–30 Hz). EEG data from 200 ms before until 800 ms after the onset of the facial stimuli were extracted. In order to discard data that was contaminated by EOG artifacts, the data were decomposed by extended infomax ICA using binica, as implemented in EEGLAB (Jung et al., 2001 (link)). Epochs with amplitude values exceeding ± 50 μV at any electrode were excluded from the average.
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10

EEG Recording and Artifact Removal

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The electroencephalogram (EEG) was recorded from 64 channels using the standard 10–20 system (Brain Products, Gilching, Germany) with an online reference to the left mastoid and an offline re-reference to the average of the left and right mastoids. The ocular artifacts of EEG data were removed by an off-line method proposed by Gratton et al. (1983) (link). The horizontal electrooculograms (EOGs) were recorded via two electrodes positioned at the outer canthi of both eyes. Vertical EOGs were recoded via two electrodes placed above and below the right eye. Recording impedance for all electrodes was held beneath 5 kΩ. Recording bandwidth was 0.01–100 Hz, at a sampling rate of 500 Hz. Signals were re-referenced offline to obtain a global average. The EEG and EOG were amplified and followed off-line. Eye movement artifacts and trials with EOG artifacts (defined as a mean EOG voltage exceeding ± 80 μV) were automatically removed. EEG data were accepted for analysis only for trials where the response for T1 and T2 was correct. The averages were then digitally filtered (0.01–30 Hz, 24 dB/octave).
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