HAuCl4·xH2O was purchased from Aladdin Chemicals. All synthetic peptides were obtained from CS Bio in Shanghai, China. We purchased chemicals from Sigma-Aldrich unless otherwise specified. Acetonitrile and water (HPLC grade) were purchased from Fisher Scientific Ltd. All products were used as received without further purification.
Hplc grade water and acetonitrile
Manufactured by Thermo Fisher Scientific
Sourced in United States
HPLC grade water and acetonitrile are high-purity solvents specifically designed for use in high-performance liquid chromatography (HPLC) applications. These solvents meet the stringent requirements for HPLC analysis, such as low levels of impurities and contaminants, to ensure accurate and reliable chromatographic results.
Automatically generated - may contain errors
Lab products found in correlation
Waters2535 chromatography system, by Waters Corporation (1 mentions)
Drx 600 spectrometer, by Bruker (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Merck Group (1 mentions)
Extra dry acetonitrile, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Gallic acid, by Thermo Fisher Scientific (1 mentions)
Acetone, by Avantor (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Concentrated hydrochloric acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Tert butyl methyl ether, by Thermo Fisher Scientific (1 mentions)
Solamargine, by MedChemExpress (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulphonic acid, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid trolox, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions)
Anhydrous ether, by Thermo Fisher Scientific (1 mentions)
Mercury 2 chloride, by Merck Group (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
5 protocols using hplc grade water and acetonitrile
1
Synthesis and Characterization of Gold Nanoparticles
2
Spectroscopic Characterization of Compounds
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NMR spectra were obtained using a Bruker DRX-600 spectrometer. HR-ESI-MS were recorded on an AB SCIE x500 Q-TOF MS spectrometer (AB Sciex Pte. Ltd., MA, United States). High-performance liquid chromatography (HPLC) was carried out using a Waters2535 chromatography system (Waters, Milford, MA, United States) equipped with a Waters 2,489 UV/visible detector with a YMC-Pack ODS-A (250 × 10 mm, 5 μm) column (YMC Co., Ltd., Kyoto, Japan). Acetonitrile and water (HPLC grade) were purchased from Fisher Scientific Ltd.
3
Fluorescent Labeling of BSA Protocol
4
Quantification of Bioactive Compounds
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Standard compounds solasodine was purchased from MP Biomedicals (Santa Ana, CA, USA) and solamargine from MedChem Express (Monmouth Junction, NJ, USA). Standards β-carotene and vitamin E (α-tocopherol), Folin Ciocalteu's phenol reagent, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and 2,2′-azinobis (3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) from Sigma–Aldrich (St. Louis, MO, USA). Gallic acid was purchased from Acros Organics (Belgium, WI, USA) and acetone from BDH Chemicals (Radnor, PA, USA). Methanol, ethyl acetate, tert-butyl methyl ether, concentrated hydrochloric acid, and HPLC grade water and acetonitrile modified with 0.1% formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA).
5
Synthesis of Fmoc-Cys(Trt) Peptide
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Fmoc-Cys (Trt) (Wang Resin LL)-resin (where Fmoc is fluorenylmethyloxycarbonyl, and Trt is triphenylmethyl), 1-hydroxy-7-azabenzotriazole (HOAT), O-(7-azabenzotriazol-1-yl)-N, N, N′, N′-tetramethyluronium hexafluorophosphate (HATU), and Fmoc-amino acids were purchased from Novabiochem (San Diego, CA, USA). Ethyl cyano(hydroxyimino)acetate (Oxyma Pure) and Fmoc-His (Boc) were purchased from CEM, Corporation (Matthews, NC, USA). Collidine (2, 4, 6-trimethylpyridine, TMP), triisopropylsilane (TIS), 3, 6-dioxa-1, 8-octanedithiol (DODT), trifluoroacetic acid (TFA), diisopropylcarbodiimide (DIPCDI), dichloromethane (DCM), and mercury(II) chloride were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mercury compounds are hazardous and should be disposed in designated chemical waste containers. HPLC grade water and acetonitrile, anhydrous ether, and N, N-dimethylformamide (DMF) were purchased from Fisher Scientific (Pittsburgh, PA, USA). Analytical and semiprep reversed-phase C-18 HPLC columns (Gemini: 300 Å, 5 µm) were purchased from Phenomenex, Inc. (Torrance, CA, USA).
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