Horseradish peroxidase hrp conjugated anti rabbit and anti mouse antibodies

Primary antibodies against caspase-3, poly(ADP-ribose) polymerase 1 (PARP-1), Akt, phospho-Akt (Ser473), phospho-Akt (Ser308), Src, pSrc (Y416), Erk, pErk (T202/204), and CD68 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CD138 was from Abcam (Cambridge, UK). APC anti-human CD68, PE mouse anti-human CD163, PE mouse anti-human BAFF, APC rat anti-human CD267 (TACI), PE anti-human CD269 (BCMA), and fluorescein isothiocyanate (FITC) mouse anti-human CD268 (BAFF receptor, or BAFF-R) were all obtained from Biolegend (San Diego, CA, USA). Primary antibodies including β-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from Sigma-Aldrich (Billerica, MA, USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were procured from Jackson ImmunoResearch Laboratories (Lancaster, PA, USA). Human BAFF antibody and mouse immunoglobulin G2b (IgG2B) isotype control were purchased from R&D Systems.

Recombinant human IL-32α (rIL-32α), Recombinant human IL-6 (rIL-6) and neutralization antibodies to human IL-6 were purchased from R&D systems (Minneapolis, MN, USA). Antibodies to human IL-32, PR3, and immunofluorescence antibody to IL-32 were purchased from Abcam (Cambridge, UK). Western blot antibodies to STAT3, pSTAT3 Ser-727, pSTAT3 Tyr-727, p38MAPK, pp38MAPK, p65 and pp65 were purchased from Cell Signaling Technology (Danvers, MA, USA). NF-κB inhibitor QNZ and STAT3 inhibitor BP-1-102 were purchased from Selleckchem (Houston, TX, USA). Primary antibody against GAPDH was purchased from Sigma-Aldrich. Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Jackson Immuno research Laboratories (West Grove, PA, USA).

Quercetin, dexamethasone, 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), propidium iodide (PI), and ribonuclease A were all purchased from Sigma(St. Louis, MO, USA) Z-VAD-FMK was purchased from R&D systems (USA). Annexin V Apoptosis Detection Kit FITC/PI was obtained from eBioscience (CA, USA). Primary antibodies against caspase-3, caspase-8, caspase-9, poly(ADP-ribose)polymerase 1 (PARP-1), and c-myc were procured from Cell Signaling Technology (MA, USA). Primary antibody against p21 was obtained from Abcam (Cambridge, UK). Primary antibody against β-actin was obtained from Sigma-Aldrich. Horseradish peroxidase (HRP)–conjugated antirabbit and antimouse antibodies were procured from Jackson Immuno Research Laboratories (PA, USA).