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Mammalian achaete scute complex like1 ascl1

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Mammalian achaete-scute complex-like1 (ASCL1) is a protein-coding gene that plays a key role in the regulation of neurogenesis and neuroendocrine cell differentiation. It is a member of the basic helix-loop-helix (bHLH) transcription factor family.

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Lab products found in correlation

P21 waf1 cip1, by Cell Signaling Technology (2 mentions) Survivin, by Cell Signaling Technology (2 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Bio-Techne (2 mentions) Total atm, by Cell Signaling Technology (2 mentions) Chromogranin a, by Zymo Research (2 mentions)

2 protocols using mammalian achaete scute complex like1 ascl1

1

Immunoblot Analysis of Cell Lysates

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Total BON cell lysates following dacarbazine ± ABT-888 treatment were prepared and analyzed by Western blotting as previously described44 (link). Each antibody was diluted as follows: 1:2000 for mammalian achaete-scute complex-like1 (ASCL1) (BD Pharmingen, San Diego, CA, USA), 1:3000 for chromogranin A (Zymed Laboratories, San Francisco, CA, USA), 1:10,000 for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Trevigen, Gaithersburg, MD, USA), and 1:1000 for p21Waf1/Cip1, cleaved poly (ADP-ribose) polymerase (PARP), phosphorylated ATM, total ATM, and Survivin (Cell Signaling Technology, Beverly, MA, USA). Antibody signals were detected using Supersignal West Femto, Dura, or Pico (Pierce, Rockford, IL, USA) chemiluminescence systems and manufacturers’ instructions were adhered to.
Somnay Y., Lubner S., Gill H., Matsumura J.B, & Chen H. (2016). The PARP inhibitor ABT-888 potentiates darbazine-induced cell death in carcinoids. Cancer gene therapy, 23(10), 348-354.
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2

Immunoblot Analysis of Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total BON cell lysates following dacarbazine ± ABT-888 treatment were prepared and analyzed by Western blotting as previously described44 (link). Each antibody was diluted as follows: 1:2000 for mammalian achaete-scute complex-like1 (ASCL1) (BD Pharmingen, San Diego, CA, USA), 1:3000 for chromogranin A (Zymed Laboratories, San Francisco, CA, USA), 1:10,000 for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Trevigen, Gaithersburg, MD, USA), and 1:1000 for p21Waf1/Cip1, cleaved poly (ADP-ribose) polymerase (PARP), phosphorylated ATM, total ATM, and Survivin (Cell Signaling Technology, Beverly, MA, USA). Antibody signals were detected using Supersignal West Femto, Dura, or Pico (Pierce, Rockford, IL, USA) chemiluminescence systems and manufacturers’ instructions were adhered to.
Somnay Y., Lubner S., Gill H., Matsumura J.B, & Chen H. (2016). The PARP inhibitor ABT-888 potentiates darbazine-induced cell death in carcinoids. Cancer gene therapy, 23(10), 348-354.
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