Perk antibody

Cerulein was purchased from MedChem Express (HY-A0190, Shanghai). Lipopolysaccharide (LPS) was purchased from Sigma (L2630, Shanghai). ABT702 was purchased from Merck Millipore (116890, Shanghai). ZM241385 (an adenosine A_2A receptor antagonist) and GSK2606414 (PERK inhibitor) were purchased from Selleck (S8105 and S7307, respectively, Shanghai). The following primary antibodies were purchased: ß-actin antibody (Proteintech, 66009), ADK antibody (Abcam, ab227087), RIP1 antibody (CST, 3,493), phosphor-RIP1 (Ser161) antibody (Affinity, AF7377), RIP3 antibody (CST, 15828), phosphor-RIP3 antibody (Abcam, ab195117), mouse MLKL antibody (Proteintech, 66675), rat MLKL antibody (Abcam, ab243142), phosphor-MLKL antibody (Affinity, AF7420), NF-κB p65 antibody (CST, 8242), phosphor-NF-κB p65 antibody (CST, 3,033), GRP78 Antibody (Proteintech, 11587), CHOP antibody (Proteintech, 15204), PERK antibody (Proteintech, 24390), phosphor-PERK antibody (CST, 3,179), eIF2α antibody (CST, 5324), phosphor-eIF2α antibody (CST, 3,398), CD11b antibody (Abcam, ab133357), MOMA-2 antibody (Abcam, ab33451), and amylase antibody (Santa Cruz Biotechnology, sc-46657). Secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States).

MC-LR with a purity of 95% was purchased from Alexis Corporation (Lausen, Switzerland). RIPA buffer, bicinchoninic acid (BCA) protein assay kit was purchased from Beyotime (Shanghai, China). The polyvinylidene fluoride (PVDF) membrane was purchased from Merck Millipore Ltd., (Billerica, MA, USA). The phosphatase inhibitor cocktail and protease inhibitor cocktail were purchased from CWBIO (Beijing, China). Trizol Reagent was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The HiScript^® II Q RT SuperMix for qRT-PCR and ChamQ Universal SYBR qRT-PCR Master Mix were purchased from Vazyme (Nanjing, China). The ERK antibody, p-ERK antibody, Raf antibody, p-Raf antibody, ZO-1 antibody, Occludin antibody, Claudin1 antibody, β-actin antibody, HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L), and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) were purchased from Proteintech (Wuhan, China).