Fluorescently labelled antibodies purchased from eBioscience (Waltham, MA) include the following: TCRβ (biotin; 1:200), TCRγδ (biotin; 1:200), and CD19 (biotin; 1:200), TCRβ (H57-597; 1:200), CD19 (eBio1D3; 1:200), TCRγδ (EbioGL3; 1:200), CD45.2 (104; 1:400), CD163 (TNKUPJ; 1:100) and IFNγ (XMG1.2; 1:100). Antibodies purchased from Biolegend (San Diego, CA) include the following: CD16/CD32 (93; 1:500), Streptavidin, CD11b(M1/70; 1:500), F4/80 (BM8; 1:200), CD206 (C068C2; 1:200), and IL-17A (TC11-18H10.1, 1:100). Antibodies purchased from BD Pharmingen (Billerica, MA) include the following: Siglec-F (E50-2440; 1:800), CD64 (X54-5/7.1; 1:200) and CD4 (RM4.5; 1:200). Antibodies purchased from Life Technologies (Washington, DC) include the following: LIVE/DEAD Fixable Blue Viability Dye (1:500). Cells were collected on an LSR Fortessa I flow cytometer equipped with FACSDIVA (BD Biosciences) software and data were analyzed with FlowJo software (Tree Star, Ashland, OR).
Lsr fortessa 1 flow cytometer
Manufactured by BD
The LSR Fortessa I flow cytometer is a high-performance instrument designed for multi-parameter analysis of cells and other biological particles. It utilizes advanced flow cytometry technology to provide precise measurement and characterization of various cellular properties, such as size, granularity, and the expression of specific markers. The LSR Fortessa I offers reliable and accurate data collection, making it a valuable tool for a wide range of applications in the field of life sciences and biomedical research.
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Lab products found in correlation
Tcrγδ, by Thermo Fisher Scientific (2 mentions)
Cd163, by Thermo Fisher Scientific (2 mentions)
Live dead fixable blue viability dye, by Thermo Fisher Scientific (2 mentions)
Cd16 cd32 93, by BioLegend (2 mentions)
Ifn γ, by Thermo Fisher Scientific (2 mentions)
Il 17a, by BioLegend (2 mentions)
Facsdiva, by BD (2 mentions)
F4 80, by BioLegend (2 mentions)
Siglec f, by BD (2 mentions)
Cd206, by BioLegend (2 mentions)
2 protocols using lsr fortessa 1 flow cytometer
1
Multiparameter Flow Cytometry Protocol
2
Multiparameter Flow Cytometry Protocol
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Fluorescently labelled antibodies purchased from eBioscience (Waltham, MA) include the following: TCRβ (biotin; 1:200), TCRγδ (biotin; 1:200), and CD19 (biotin; 1:200), TCRβ (H57-597; 1:200), CD19 (eBio1D3; 1:200), TCRγδ (EbioGL3; 1:200), CD45.2 (104; 1:400), CD163 (TNKUPJ; 1:100) and IFNγ (XMG1.2; 1:100). Antibodies purchased from Biolegend (San Diego, CA) include the following: CD16/CD32 (93; 1:500), Streptavidin, CD11b(M1/70; 1:500), F4/80 (BM8; 1:200), CD206 (C068C2; 1:200), and IL-17A (TC11-18H10.1, 1:100). Antibodies purchased from BD Pharmingen (Billerica, MA) include the following: Siglec-F (E50-2440; 1:800), CD64 (X54-5/7.1; 1:200) and CD4 (RM4.5; 1:200). Antibodies purchased from Life Technologies (Washington, DC) include the following: LIVE/DEAD Fixable Blue Viability Dye (1:500). Cells were collected on an LSR Fortessa I flow cytometer equipped with FACSDIVA (BD Biosciences) software and data were analyzed with FlowJo software (Tree Star, Ashland, OR).
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