All studies were approved by the Human Research Protection program at the University of California, San Diego, and were conducted in adherence to the Declaration of Helsinki Principles. Written, informed consent was obtained from all donors for all procedures. Skin biopsy samples were obtained from normal skin and lesional and non-lesional skin of AD patients, frozen in OCT, sectioned using a cryotome, and stored on microscope slides at −80°C until use. Briefly, cells were fixed with cold acetone, and stained for the presence of MARCO using a monoclonal antibody (a kind gift from Lester Kobzik, Harvard University, MA) and Keratin-14 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and Alexa-fluor 488- and 568-labeled secondary antibodies, respectively (Life Technologies, Grand Island, NY) and counterstained with DAPI. Images were captured using a BX41 microscope (Olympus, Center Valley, PA).
Alexa fluor 488 and 568 labeled secondary antibodies
Manufactured by Thermo Fisher Scientific
Alexa-Fluor 488- and 568-labeled secondary antibodies are fluorescently-tagged antibodies used for sensitive detection and visualization of target proteins in various applications, such as immunofluorescence, flow cytometry, and Western blotting. These antibodies are designed to bind and detect primary antibodies, amplifying the fluorescent signal for improved target identification and localization.
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Lab products found in correlation
2 protocols using alexa fluor 488 and 568 labeled secondary antibodies
1
Immunostaining of Skin Biopsies in AD
2
Immunostaining of Skin Biopsies in AD
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All studies were approved by the Human Research Protection program at the University of California, San Diego, and were conducted in adherence to the Declaration of Helsinki Principles. Written, informed consent was obtained from all donors for all procedures. Skin biopsy samples were obtained from normal skin and lesional and non-lesional skin of AD patients, frozen in OCT, sectioned using a cryotome, and stored on microscope slides at −80°C until use. Briefly, cells were fixed with cold acetone, and stained for the presence of MARCO using a monoclonal antibody (a kind gift from Lester Kobzik, Harvard University, MA) and Keratin-14 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and Alexa-fluor 488- and 568-labeled secondary antibodies, respectively (Life Technologies, Grand Island, NY) and counterstained with DAPI. Images were captured using a BX41 microscope (Olympus, Center Valley, PA).
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