Anti phospho eif2α ab32157

Manufactured by Abcam

Anti-phospho-eIF2α (ab32157) is a primary antibody that recognizes the phosphorylated form of the eukaryotic translation initiation factor 2 alpha (eIF2α) protein. eIF2α plays a critical role in the regulation of protein synthesis. Phosphorylation of eIF2α is a key event in the cellular response to various stress stimuli.

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Lab products found in correlation

Anti gapdh 2118, by Cell Signaling Technology (1 mentions) Bapta am b1205, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Tunicamycin t7765, by Merck Group (1 mentions) Anti iκb α sc 1643, by Santa Cruz Biotechnology (1 mentions) Anti nf κb sc 8008, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti phospho eif2α ab32157

Antibodies for Protein Expression Analysis

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Antibodies against eIF4G1 (A300-502A), eIF4G2 (A302-249A), eIF3b/EIF3S9 (A301-761A), eIF3d/EIF3S7 (A301-758A), GAPDH (A300-639A) were purchased from Bethyl laboratories. Anti-phospho-eIF2α (ab32157) were from Abcam. Anti-eIF4E-BP1 (9452) and anti-phospho-eIF4E-BP1 (Thr37/46) (2855) were from Cell Signaling Technology. HRP-conjugated secondary antibodies were from Invitrogen (anti-rabbit 31460, anti-mouse 31431).

Smirnova V.V., Shestakova E.D., Nogina D.S., Mishchenko P.A., Prikazchikova T.A., Zatsepin T.S., Kulakovskiy I.V., Shatsky I.N, & Terenin I.M. (2022). Ribosomal leaky scanning through a translated uORF requires eIF4G2. Nucleic Acids Research, 50(2), 1111-1127.

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Immunoblotting and Immunofluorescence Antibodies for Cell Signaling

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Anti-PC2 (sc-47734; for immunoblotting), anti-NFκB (sc-8008), and anti-IκBα (sc-1643) antibodies were purchased from Santa Cruz Biotechnology. Anti-PC2 antibody for immunofluorescent imaging was a kind gift from Dr. Stefan Somlo (Yale). Mouse anti-beta-Actin (#3700), rabbit anti-beta-Actin (#4970), anti-CHOP (#2895), anti-cleaved caspase-3 (#9664), and anti-GAPDH (#2118) antibodies were purchased from Cell Signaling Technology. Anti-4-Hydroxynonenal (ab48506), anti-eIF2α (ab50733), anti-phospho-eIF2α (ab32157) antibodies, and kainic acid (ab120100) were purchased from Abcam. Anti-VDAC (PA1-954A) antibody was purchased from Thermo Fisher Scientific. Hydrogen peroxide (H325-100) and BAPTA-AM (B1205) were purchased from Thermo Fisher Scientific. Tunicamycin (T7765), and Dimethyl sulfoxide (DMSO; W387520) were purchased from Sigma-Aldrich.

Brill A.L., Fischer T.T., Walters J.M., Marlier A., Sewanan L.R., Wilson P.C., Johnson E.K., Moeckel G., Cantley L.G., Campbell S.G., Nerbonne J.M., Chung H.J., Robert M.E, & Ehrlich B.E. (2020). Polycystin 2 is increased in disease to protect against stress-induced cell death. Scientific Reports, 10, 386.

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