2 propanol optima lc ms grade

Acetonitrile, methanol, and 2-propanol (Optima LC-MS grade) were purchased from Fisher Chemical(Waltham, USA); methyl-tert-butyl ether (MTBE), ammonium formate, ammonium hydrogen carbonate (BioUltra grade) and chloroform (analytical reagent grade) were purchased from Sigma-Aldrich (St. Louis, USA) Deionized water was obtained from an in-house water purification system. The following standards, lysophosphatidylethanolamine (LPE) 14:0, Lysophosphatidylcholine (LPC) 13:0, phosphatidylcholine (PC) 13:0_13:0, phosphatidylethanolamine (PE) 14:0_14:0, phosphatidylserine (PS) 14:0_14:0, phosphatidylinositol (PI) 12:0–13:0, ceramide d18:1/17:0, glucosyl(ß) C8 ceramide, hexosyl1ceramide d18:1/8:0 and 06:0 sphingomyelin (SM) were purchased from Avanti Polar Lipids (Alabaster, USA). GM3 d18:1/18:0 d3 was purchased from Matreya LLC (State College, USA). Diacylglycerol (DG) 15:0_15:0 was purchased from Santa Cruz Biotechnology, Inc. (Dallas, USA). Glyceryl triheptadecanoate (TAG) 17:0_17:0_17:0 was purchased from Sigma Aldrich (St. Louis, USA) and glyceryl trihexadecanoate (TAG) 48:0 d5 was purchased from CDN Isotopes (Pointe Claire, Canada)

Organic solvents used were as follows: acetonitrile, heptane, and 2-propanol Optima™ LC/MS grade were supplied by Fisher Chemical. Ultrapure water was produced using the Milli-Q Plus 185 system (Millipore, Billerica, MA, United States). O-methoxyamine hydrochloride, sialylation-grade pyridine, BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) with 1% TMCS (trimethylchlorosilane), C8–C40 alkane calibration standard mix, C:18 methyl ester, and tricosane analytical standard were purchased from Sigma-Aldrich (St. Louis, MO, United States).