Guinea pig anti pv

Manufactured by Synaptic Systems

Sourced in United States

Guinea pig anti-PV is a laboratory reagent used in research applications. It is an antibody raised in guinea pigs that specifically binds to the PV (parvalbumin) protein. The core function of this product is to facilitate the detection and study of the PV protein in biological samples.

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Lab products found in correlation

Axio imager m2, by Zeiss (1 mentions) Lsm 710, by Zeiss (1 mentions) Rabbit anti camkii, by Abcam (1 mentions) Guinea pig anti cr, by Synaptic Systems (1 mentions) Plan apo vc 20 objective, by Nikon (1 mentions) Rabbit anti vip, by Immunostar (1 mentions) Oct medium, by Leica (1 mentions) Sp2 confocal microscope, by Leica (1 mentions) Cryostat, by Leica (1 mentions) Rabbit anti th, by Merck Group (1 mentions) Mouse anti nf200, by Merck Group (1 mentions)

Close spelling variants of this product

Guinea Pig anti-PV antibody (2 citations)

3 protocols using guinea pig anti pv

Immunohistochemical Profiling of Fear-Conditioned Neurons

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Brains from fear conditioned animals were cut into 60 μm-thick coronal sections. Sections were stained with the following primary antibodies: guinea pig anti-PV (1:2000, Synaptic Systems) and rabbit anti-c-Fos antibodies (1:500, Abcam).
Recorded interneurons were stained with Cy3-conjugated (Life Technologies) or Alexa488-conjugated (Invitrogen) streptavidin (1:1000-3000). Somatic immunoreactivity was assessed using guinea pig anti-PV (1:2000, Synaptic Systems) or goat anti-PV (1:500, Abcam) primary antibodies. Immunoreactivity of the recorded neurons was visualized using an epifluorescence microscope (AxioImager M2, Zeiss) or a laser-scanning confocal microscope (LSM 710, Zeiss). For a detailed description of immunohistochemical procedures see Supplementary materials and methods.

Bocchio M., Fucsina G., Oikonomidis L., McHugh S.B., Bannerman D.M., Sharp T, & Capogna M. (2015). Increased Serotonin Transporter Expression Reduces Fear and Recruitment of Parvalbumin Interneurons of the Amygdala. Neuropsychopharmacology, 40(13), 3015-3026.

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Immunohistochemical Analysis of Inhibitory Interneuron Subpopulations

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Some slices prepared from AAV-injected VIP-IRES-cre::GAD65-EGFP mice were fixed in 4% PFA in 0.1 m PB and after overnight incubation were resectioned at a 40 μm thickness. Different sections were processed for single immunostaining with rabbit anti-pro-CCK (Frontiers Institute; 1:1000), guinea pig anti-NPY (Synaptic Systems; 1:1000), or rabbit anti-CaMKII (Abcam; 1:250). Visualization was performed with Cy5-conjugated secondary antibodies. In addition, double immunostaining was obtained using a mixture of rat anti-SOM (Millipore; 1:500) and guinea pig anti-PV (Synaptic Systems; 1:1000) antibodies or a mixture of rabbit anti-VIP (Immunostar; 1:4000) and guinea pig anti-CR (Synaptic Systems; 1:1000) antibodies. Here, DyL405-conjugated and Cy5-conjugated appropriate secondary antibodies were used to visualize the antigen–antibody complexes. Confocal images were obtained with a Nikon C2 confocal microscope (Plan Apo VC 20× objective; numerical aperture, 0.75; z-step size, 2 μm; xy, 0.62 μm/pixel) and the analysis was conducted using Neurolucida Explorer (RRID:SCR_001775). Analysis of the colocalization between CCK and EGFP was limited to neurons with large soma, as these are known to be CB1+ basket cells (Szabó et al., 2014 (link)) and we did not find overlap between CCK and VIP in these cells in this mouse line. In addition, data for VIP+ and CR+ INs were combined.

Rhomberg T., Rovira-Esteban L., Vikór A., Paradiso E., Kremser C., Nagy-Pál P., Papp O.I., Tasan R., Erdélyi F., Szabó G., Ferraguti F, & Hájos N. (2018). Vasoactive Intestinal Polypeptide-Immunoreactive Interneurons within Circuits of the Mouse Basolateral Amygdala. The Journal of Neuroscience, 38(31), 6983-7003.

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Immunohistochemical Analysis of DRG and Skin

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DRG and skin (hairy and glabrous) were dissected and fixed with 4% PFA for 2 h or O/N 4°C, respectively. Tissues were cryoprotected in 30% sucrose in PBS O/N at 4°C, then embedded in OCT medium (Leica). Both DRG and skin were sectioned at a thickness of 40 μm using a cryostat (Leica). Sections were then treated with cold 50% ethanol for 30 min, followed by 2% BSA-0.3% Triton-X in PBS blocking solution for 1 h at RT, and finally incubated with primary antibodies in blocking solution O/N at 4°C. Secondary antibodies were diluted in blocking solution and incubated for 2 h at RT. Sections were mounted using DAPI-Fluoroshield™ Medium (Sigma). All images were acquired at 40× with a Leica SP2 confocal microscope and analyzed with ImageJ-Fiji (NIH, MD, 312 USA).
The following primary antibodies were used: 1:300 mouse anti-TRPV1 (Neuromics), 1:50 mouse anti-TrkA (MNAC13, 5.4 μg/μl) (105 (link)), 1:200 mouse anti-CGRP (Immunostar), 1:100 isolectin GS-B4-biotin conjugate (Invitrogen), 1:300 guinea pig anti-PV (Synaptic Systems), 1:200 rabbit anti-TH (Millipore), 1:500 mouse anti-NF200 (Sigma), 1:200 rabbit anti-PGP9.5 (Dako-Agilent), 1:300 guinea pig anti-VAChT (Synaptic System). All Alexa-conjugated secondary antibodies were used at 1:1000 concentration.

Pacifico P., Testa G., Amodeo R., Mainardi M., Tiberi A., Convertino D., Arevalo J.C., Marchetti L., Costa M., Cattaneo A, & Capsoni S. (2022). Human TrkAR649W mutation impairs nociception, sweating and cognitive abilities: a mouse model of HSAN IV. Human Molecular Genetics, 32(8), 1380-1400.

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