Acs grade acetone

A. niger fungi were obtained from domestic cat hair samples and cultured on an agar medium. D-Glucosamine hydrochloride was obtained from MP Biomedicals (Santa Ana, CA). Chitin polymer (100% acetylated) and N-acetyl-D-glucosamine (> 98.0%) were obtained from TCI (Portland, OR). Chitin polymer (20–30% deacetylated) was obtained from Alfa Aesar. ACS-grade acetone and Optima LC-MS grade formic acid were obtained from Fisher Chemical (Waltham, MA). Hydrochloric acid was obtained from Ward’s Science (Rochester, NY). LC-MS grade water, LC-MS grade acetonitrile, low molecular weight chitosan (96% deacetylated), and ammonium acetate (> 98.0%) were obtained were obtained from EMD Millipore (Burlington, MA). 0.2-μm Captiva Econofilters were obtained from Agilent (Palo Alto, CA). C18 Macro spin columns were obtained from Harvard Apparatus (Holliston, MA).

Optima HPLC grade methanol, Optima HPLC grade water, Optima HPLC grade acetonitrile, American Chemical Society (ACS) grade acetone, ACS grade methanol, ACS grade pentane, hydrochloric acid, ammonium acetate, dipotassium phosphate, monopotassium phosphate, magnesium chloride (MgCl₂) and reduced glutathione (GSH) were purchased from Fisher Chemical (Fair Lawn, NJ, USA); NADPH, 1-aminobenzotriazole, methimazole, 1-naphthol and hydroxyacetone from Acros Organics (Morris Plain, NJ, USA); UDPGA, saccharolactone and 2,4-dichlorophenoxyacetic acid from Sigma-Aldrich (St. Louis, MO, USA); bupropion, benzydamine and alamethicin from Alfa Aesar (Ward Hill, MA, USA); oxcarbazepine from European Pharmacopoeia Reference Standard (Strasbourg, France); ticlopidine from Tokyo Chemical Industry (Tokyo, Japan); hydroxybupropion from Cerilliant Corporation (Round Rock, Texas, USA); deuterated acetone (acetone-d₆) from Cambridge Isotope Labs (Cambridge, MA, USA); hydrogen peroxide (50%) from Univar (Redmond, WA, USA); HLM, rat liver microsomes (RLM), DLM and human lung microsomes (HLungM) from Sekisui XenoTech (Kansas City, KS, USA); human recombinant CYP (rCYP) bactosomes expressed in Escherichia coli (E. coli) from Cypex (Dundee, Scotland); human recombinant flavin monooxygenase (rFMO) supersomes and human recombinant UGT (rUGT) supersomes expressed in insect cells from Corning (Woburn, MA, USA).