Peridinin chlorophyll protein percp conjugated anti human cd56

Freshly thawed PBMC were used for surface and intracellular staining regarding flow cytometric analysis. The following monoclonal antibodies were used: Brilliant Violet (BV421)-conjugated anti-human PD-L1 (BD Biosciences), BV510-conjugated anti-human CD3 (BD Biosciences), fluorescein isothiocyanate (FITC)-conjugated anti-human CD4 (BD Biosciences), FITC-conjugated anti-human CD14 (BD Biosciences), (phycoerythrin (PE)-conjugated anti-human PD-1 (Beckmann-Coulter), PE-conjugated anti-human Gal-9 (Biolegend), Peridinin Chlorophyll Protein (PerCP)-conjugated anti-human CD56 (BD Biosciences), PerCP-conjugated anti-human Perforin (BD Biosciences), allophycocyanin (APC)-conjugated anti-human TIM-3 (R&D Systems), APC-conjugated anti-human CD56 (BD Biosciences), APC-conjugated anti-human FoxP3 (eBioscience), APC/H7-conjugated anti-human CD8 (BD Biosciences).

Freshly thawed PBMC and PTL samples were used for surface and intracellular immuno-labeling and flow cytometric analysis. The following antibodies were used: fluorescein isothiocyanate (FITC)-conjugated anti-human CD4 (Clone: RPA-T4, BD Biosciences, Franklin Lakes, NJ, USA), phycoerythrin (PE)-conjugated anti-human Gal-9 (Clone: 9M1–3, Biolegend, San Diego, CA, USA), PE-conjugated anti-human TIM-3 (Clone: 344823, R&D Systems, Minneapolis, MN, USA), Peridinin-chlorophyll protein (PerCP)-conjugated anti-human CD56 (Clone: B159, BD Biosciences Franklin Lakes, NJ, USA), allophycocyanin (APC)-conjugated anti-human TIM-3 (Clone: 344823, R&D Systems, Minneapolis, MN, USA), APC-conjugated anti-human FoxP3 (Clone: 236A/E7, eBioscience, Santa Clara, CA, USA), APC-H7 conjugated anti-human CD8 (Clone: SK1, BD Biosciences, Franklin Lakes, NJ, USA) and Brilliant Violet (BV) 510-conjugated anti-human CD3 (Clone: UCHT1, BD Biosciences, Franklin Lakes, NJ, USA). Control antibodies included isotype-matched FITC-, PE-, APC-, APC-H7, or BV510-conjugated mouse antibodies.