Sc 365521

Details of the specific antibodies that were used in the study are as follows: anti-CEP128 (HPA001116, Sigma-Aldrich, WB: 1:1000, IF: 1:200); anti-Ac-Tubulin (ab24610, abcam, IF: 1:1000); anti-NIN (A8215, ABclonal, WB: 1:1000); anti-CEP170 (27325-1-AP, Proteintch, WB: 1:500); anti-YY1 (sc-7341, Santa Cruz Biotechnology, WB: 1:500); anti-GR-β (sc-393232, Santa Cruz Biotechnology, WB: 1:500); anti-SEPT4 (Septin 4) (A10238, ABclonal, IF: 1:100); anti-RCBTB2 (13225-1-AP, Proteintech, WB: 1:1000, IF: 1:50); anti-WBP2NL (22587-1-AP, Proteintech, WB: 1:500, IF: 1:50); anti-CRISP1(MAB4675, R&D Systems, WB: 1:500, IF: 1:50); anti-PRSS55 (bs-19443R, Bioss, WB: 1:1000, IF: 1:50); anti-ubiquitin (ab7780, Abcam, WB: 1:500), anti-GAPDH (ab8245, Abcam, WB: 1:1000); anti-CEBPβ (sc-7962, Santa Cruz Biotechnology, WB: 1:500); anti-Centriolin (sc-365521, Santa Cruz Biotechnology, WB: 1:500).

The following antibodies were used for localization studies and Western blot: ALMS1 (1:1500–2000, rb, ab84892 Abcam; 1:500, rb, NB100-97823, Novusbio), ARL13B (1:200, ms, 73-287, Neuromab; rb, 17711-1-AP, Proteintech); TUBGCP2 (1:100, rb, 25856-1-AP, Proteintech), CEP70 (1:500, rb, ab227456, Abcam), acetylated tubulin (1:250, ms, ab24610, Abcam), CEP250 (1:200, rb, 14498-1-AP, Proteintech), Centrin-3 (1:500, rb, PA5-35865, Thermo), Centriolin (1:200, ms, sc-365521, Santa Cruz), RPGR (1:500, rb, HPA001593, Sigma Aldrich). Secondary Antibodies were Alexa488 goat anti-mouse (A11029, Molecular probes) and anti-rabbit (A11034, Molecular Probes), Alexa568 goat anti-mouse (A11031, Molecular probes) and anti-rabbit (A11036, Molecular Probes). For Western blot FLAGM2-HRP (Sigma) was used.