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7 protocols using lumogen f red 305

1

Preparation and Purification of PLGA-CIP Nanoparticles

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Blank PLGA and CIP-loaded PLGA NP (PLGA/CIP NP) were prepared by employing the nanoprecipitation methodology, as previously described by Günday et al. [52 ]. In brief, the solvent system (consisting of 0.4 % (w/v) PLGA and 0.15 % (w/v) Span 80® solution in acetone, mixed with 4.85 % CIP solution in oleic acid) and the non-solvent system (0.15 % (w/v) aqueous Tween 80 solution) were nanoprecipitated continuously at a 1:2 ratio and an angle of 180° under ambient conditions. In case of the labelled NP preparation, 1.12 × 10−4 % (w/v) Lumogen F Red 305® (BASF, Germany) was introduced into the solvent system. The purification process, involving the removal of free formulation components and organic solvent, as well as the concentration of the formulation to achieve the desired CIP concentration, was achieved through continuous flow filtration using modified polyethersulfone MidiKros® hollow fiber filters (MWCO: 10 kDa, surface area: 235 cm2; Spectrum, Repligen Europe B.V., Netherlands).
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2

Photosensitizer-based Reactor Fabrication

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Ethanol (purity (V/V) ≥ 99.5%) and
diethyl ether (technical) were purchased from VWR Chemicals. Acetonitrile
(AR, Biosolve Chimie SARL) and methylene blue were bought from Merck
KGaA. Lumogen F red 305 and 9,10-diphenylanthracene (>95.0%) were
acquired from BASF and TCI, respectively. Sylgard 184 silicone elastomer
(base + curing agent) was purchased from Dow Corning.
For the
3D printing of the reactor molds, high-impact polystyrene filament
(ø1.75 mm, neutral, Apollo series) was obtained from webshop
123-3D.nl; polystyrene plates (black, 270 length × 260 width
× 3 thickness mm3) were provided by S-POLYTEC GmbH.
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3

Antimicrobial Paper Coating Development

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Cellulose acetate (CA) (Sigma-Aldrich, St. Louis, MO, USA, Mn 50,000 g/mol, DS 2.5), Triclosan (Alfa Aesar, Kandel, Germany, 99%), 4-Hexylresorcinol (Sigma-Aldrich, St. Louis, MO, USA, 98%), acetone (Brenntag, Essen, Germany, technical grade), Lumogen® F Red 305 (BASF, Ludwigshafen, Germany), Inhibition plate (Axonlab, Reichenbach, Germany), Bacillus subtilis, pH 6.6), Rotilabo® filter paper Type 601 (Carl ROTH, Karlsruhe, Germany) were used as model paper substrate, deionized water was used in all experiments.
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4

Fluorescent Polymer Coatings for Luminescent Solar Concentrators

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All materials employed in this study are of commercial source and were used as received. Lumogen F Red 305 (from now on referred to as LR305, BASF) was used as the fluorescent doping species in all polymeric coatings presented in this work. PMMA (Perspex XT, Lucite) was used as polymeric carrier in reference luminescent coatings. Solef 6010 poly(vinylidenefluoride) – PVDF, Solef 31508 (a poly(vinylidenefluoride-co-chlorotrifluoroethylene) copolymer), and Solef 11005 (a poly(vinylidenefluoride-co-hexafluoropropylene-co-hydroxyethylacrylate) terpolymer) were produced and supplied by Solvay Specialty Polymers. Chloroform (CHCl3), toluene and N-methylpyrrolidone (NMP) were purchased by Sigma Aldrich and used as solvents for the fabrication of LSC devices.
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5

HER2 Protein Detection Assay

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Emulgen (Kao Corporation, Tokyo,
Japan), 1,3-bis(hydroxymethyl)urea (Tokyo Chemical Industry Co., Ltd.,
Tokyo, Japan), methylol melamine (Nippon Carbide Industries Co., Inc.,
Tokyo, Japan), dodecylbenzenesulfonic acid (Kanto Chemical Co., Inc.,
Tokyo, Japan), Lumogen F Red 305 (BASF AG, Ludwigshafen, Germany),
dodecylbenzenesulfonic acid sodium salt (Kanto Chemical), CMD (CMD-L,
molecular weight: 10 kDa, Meito Sangyo Co., Ltd., Nagoya, Japan),
NHS (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), EDC (Tokyo
Chemical Industry), SA (ProSpec-Tany TechnoGene Ltd., Ness Ziona,
Israel), rabbit anti-HER2 IgG (clone: EP1045Y, abcam, Cambridge, U.K.),
biotinylated anti-rabbit antibody (DISCOVERY Universal Secondary Antibody,
F. Hoffmann-La Roche Ltd., Basel, Switzerland), horseradish peroxidase-labeled
anti-rabbit antibody (Histofine Simple Stain MAX PO (MULTI), Nichirei
Biosciences Inc., Tokyo, Japan), 3,3′-diaminobenzidine (DAB,
FUJIFILM Wako Pure Chemical), and all other reagents were analytical
grade and used without further purification. Ultrapure water (UPW)
used during the experiment was produced using a Millipore Milli-Q
water purification system (Burlington, MA).
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6

Biochemical Composition Analysis of Bevacizumab Formulations

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Human serum albumin, sodium deoxycholate (DS), docusate sodium salt (DOCU), poly(ethylene glycol) 35,000 Da (PEG35), trehalose dihydrate, sodium azide, and agarose were purchased from Sigma-Aldrich (Steinheim, Germany). Bevacizumab (Avastin®) was purchased from Roche (Madrid, Spain). Ethanol absolute was obtained from Scharlab (Sentmenat, Spain). Lumogen® F-Red 305 was supplied by BASF (Ludwigshafen am Rhein, Germany). European bacteriological agar, peptone, LB broth, and agarose were provided by Condalab (Torrejón de Ardoz, Spain). O.C.T.™ Compound Tissue-Tek was obtained from Sakura Finetek Europe (Alphen aan Der Rijn, The Netherlands). Isoflurane was purchased from Braun (Barcelona, Spain). Shikari® Q-BEVA Enzyme Immunoassay used for the detection of bevacizumab was purchased from Matriks Biotek (Gölbaşı, Turkey).
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7

Synthesis of Luminescent Silole Derivatives

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Palladium on carbon (10 wt%), bis(2-aminopropyl) polypropylene glycol-block-poly-ethylene glycol-block-polypropylene glycol (Jeffamine ED-600, Mw = 600 g mol−1) and 3-(triethoxysilyl)propylisocyanate (ICPTES, 95.0%) were purchased from Sigma-Aldrich. Sodium borohydride (NaBH4, 98%), tetrahydrofuran (THF, ≥99.9%), ethanol (EtOH, 95.0%), and hydrochloric acid (HCl, 37%) were obtained from Fisher Scientific. Dry dichloromethane (CH2Cl2) and THF were obtained using a solvent purification system, PureSolve MD5 purchased from Inert Technology (Amesbury, MA, USA). Water was obtained from a Millipore Simpak 2 water purification system (ρ = 18 mΩ). Lumogen F Red 305 (LR305) was a kind gift from BASF® Germany. All materials were used as received. DMTPS56 (link) and 1,1-dimethyl-2,5-di(4-nitrophenyl)-3,4-diphenylsilole (DMTPS-NO2)57 (link) were prepared according to literature procedures.
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