Igf2bp2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

IGF2BP2 is a protein that binds to insulin-like growth factor 2 mRNA and plays a role in its localization, stability, and translation. It is involved in various cellular processes, including cell growth, differentiation, and development.

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Lab products found in correlation

Cpt1a, by Abcam (1 mentions) Ct oatp1b3, by Abcam (1 mentions) Ndufa2, by Abcam (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Boster Bio (1 mentions) Murf1, by Santa Cruz Biotechnology (1 mentions) Mafbx, by Santa Cruz Biotechnology (1 mentions) Cyclin e, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg, by Boster Bio (1 mentions) β tubulin, by Santa Cruz Biotechnology (1 mentions) Cyclin d1, by Santa Cruz Biotechnology (1 mentions)

3 protocols using igf2bp2

Immunohistochemical Profiling of Cancer Markers

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The de-paraffinized sections were incubated with 20% goat serum for 30 min and were incubated with primary antibodies against Ct-OATP1B3 (1:200, Abcam, USA), IGF2BP2 (1:100, Santa Cruz, USA), CPT1A (1:1000, Abcam, USA), and NDUFA2 (1:100, Abcam, USA) at 4 °C overnight followed by the secondary antibody for 1 h at 37 °C. The calculation method of IHC score was performed as described previously [49 (link)]. Ct-OATP1B3 expression level was analyzed by classifying IHC score as low (based on the IHC score lower than the median value) and as high (based on an IHC score higher than the median value).

Huang Y., Du Y., Zheng Y., Wen C., Zou H., Huang J., Zhou H., Zhao H, & Wu L. (2022). Ct-OATP1B3 promotes high-grade serous ovarian cancer metastasis by regulation of fatty acid beta-oxidation and oxidative phosphorylation. Cell Death & Disease, 13(6), 556.

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Silencing Alkbh5 and Igf2bp2 in Cells and Oocytes

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Small interference RNA (siRNA) of murine Alkbh5 and Igf2bp2 were purchased from Santa Cruz (sc-141022) and GenePharma respectively. For gene knockdown in cell lines, mGC was transfected with siRNA via Lipofectamine™ RNAiMAX (Invitrogen, 13778150). Protein analysis was performed 48 h after transfection. For RNA interference in oocytes, approximately 5–10 pl siRNA diluted with RNase-free water (60 μM) was microinjected into GV oocytes as detailed in the followings.

Bai L., Xiang Y., Tang M., Liu S., Chen Q., Chen Q., Zhang M., Wan S., Sang Y., Li Q., Wang S., Li Z., Song Y., Hu X., Mao L., Feng G., Cui L., Ye Y, & Zhu Y. (2023). ALKBH5 controls the meiosis-coupled mRNA clearance in oocytes by removing the N 6-methyladenosine methylation. Nature Communications, 14, 6532.

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Comprehensive Immunoblotting Analysis of Muscle Proteins

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Total protein of the cell or tissue samples was harvested using radioimmunoprecipitation lysis buffer after washing three times with PBS. Immunoblotting was carried out according to our previous method (10 (link)). The primary antibodies targeted the following proteins: MyHC (1:500; no. MAB4470; R and D Systems), MyoD (1:500; no. NB100-56511; Novus Biologicals), AKT (1:1000; no. 9272S; CST), p-AKT (Ser473) (1:2000; no. 4060S; CST), PI3K (p55α) (1:1000; no. 11889S; CST), p-PI3K p55 (Tyr199) (1:1000; no. 4228S; CST), β-tubulin (1:200; no. sc-58880; Santa Cruz Biotechnology), MyoG (1:1000; no. NB100-56510SS; Novus Biologicals), MuRF1 (1:200; no. sc-398608; Santa Cruz Biotechnology), MAFbx (1:200; no. sc-166806, Santa Cruz Biotechnology), cyclin E (1:100; no.sc-377100; Santa Cruz Biotechnology), cyclin D1 (1:100; no. sc-450; Santa Cruz Biotechnology), PTEN (1:500; no. sc-7974; Santa Cruz Biotechnology), IGF2BP2 (1:100; no. sc-377014; Santa Cruz Biotechnology), and proliferating cell nuclear antigen (1:100; no. sc-56; Santa Cruz Biotechnology). The secondary antibodies included goat anti-rabbit IgG (1:3000; no. BA1054; BosterBio) and goat antimouse IgG (1:3000; no. BA1050; BosterBio).

Cai R., Zhang Q., Wang Y., Yong W., Zhao R, & Pang W. (2021). Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis. The Journal of Biological Chemistry, 296, 100376.

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