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Irf 1 sirnas

Manufactured by RiboBio
Sourced in China

IRF-1 siRNAs are short, double-stranded RNA molecules designed to target and silence the expression of the interferon regulatory factor-1 (IRF-1) gene. IRF-1 is a transcription factor that plays a role in regulating the expression of various genes involved in immune response and cellular processes. The core function of IRF-1 siRNAs is to knockdown IRF-1 gene expression, which can be used for research purposes to study the role of IRF-1 in different biological systems.

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2 protocols using irf 1 sirnas

1

Investigating IRF-1 Regulation in Cellular Processes

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Dulbecco’s modified eagle’s medium/F-12 (DMEM/F-12, 12400) and fetal bovine serum (FBS, 10099) were purchased from Gibco. AdIRF-1 (adenoviruses encoding murine IRF-1) and AdGFP (empty adenoviral vector control) were purchased from Hanbio. IRF-1 siRNAs and siRNA-NC (negative control) were purchased from RiboBio Co., Ltd. (2131). SB203580 (S1076) was purchased from SelleckInc. Trizol (9108) and lipofectamineTM 3000 (11668) were obtained from Invitrogen. Rabbit Abs specific for IRF-1 (8478), P38 (8690), p-P38 (4511), LC3 (12741), caspase-3 (9663), cleaved caspase-3 (9664) and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (7074) were purchased from Cell Signaling Technology Inc. Anti-P62 Abs (5114) for immunoblotting were from Cell Signaling Technology Inc and anti-P62 Abs (BA2849) for immunostaining were from Boster. Anti-PCNA Ab (BS5842) was from Bioworld Technology Inc. Anti-GAPDH Ab (TA-08) was from ZSGB-BIO.
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2

Glycyrrhizin Acid Modulates IRF-1 and Autophagy

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Dulbecco's modified eagle's medium/F-12 (DMEM/F-12) and fetal bovine serum (FBS) were purchased from Gibco. Glycyrrhizin Acid (GA) was obtained from Xi'an Haoxuan Biotechnology Co. Ltd., (Xi'an, China) IRF-1 siRNAs and siRNA-NC (negative control) were purchased from RiboBio Co., Ltd.. Trizol and lipofectamine TM 3000 were obtained from Invitrogen. Rabbit Abs specific for IRF-1, microtubule-associated protein 1 light chain 3 (LC3), Bcl-2, Beclin 1, HMGB1, β-actin and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were purchased from Cell Signaling Technology Inc.
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