For phenotypic analysis of CD4+-T, the following antibodies were used: CD4 PE (Clone RPA-T4), IFN-γ FITC (Clone B27), HLA-DR allophycocyanin (APC) (clone G46-6) (BD Pharmingen; BD Biosciences), CD3 PerCP (clone SK7) (Becton Dickinson, Franklin Lakes, NJ, USA); CD4 PB (Clone RPA-T4), CD8 PB (Clone RPA-T8), CD45RA PE/Cy7 (clone H100), CD25 PE (clone M-A251), CD127 PerCP/Cy5.5 (clone A019D5), CD185 APC/Cy7 (clone J252D4) (Biolegend); and CD54 Pacific Blue (clone HCD54), IL-17 efluor660 (eBioscience). Intracellular staining of FoxP3 was carried out with the anti-Human Foxp3 Staining Set APC (clone 236A/E7) (eBioscience). Flow cytometry was carried out on a FACS Canto II (BD Biosciences).
Anti human foxp3 staining set apc clone 236a e7
Manufactured by Thermo Fisher Scientific
The Anti-Human Foxp3 Staining Set APC (clone 236A/E7) is a laboratory reagent used for the detection and analysis of Foxp3-expressing cells. Foxp3 is a transcription factor that is primarily expressed in regulatory T cells (Tregs). The set includes an anti-Foxp3 antibody conjugated with the fluorescent dye APC, which can be used in flow cytometry applications to identify and quantify Foxp3-positive cells within a sample.
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Lab products found in correlation
Facscanto 2, by BD (2 mentions)
Ifn γ fitc, by BD (1 mentions)
Cd4 pb, by BioLegend (1 mentions)
Cd8 pb, by BioLegend (1 mentions)
Hla dr allophycocyanin apc clone g46 6, by BD (1 mentions)
Cd25 pe clone m a251, by BioLegend (1 mentions)
Cd4 pe, by BD (1 mentions)
Apc cy7, by BioLegend (1 mentions)
Percp cy5, by BioLegend (1 mentions)
Cd25 pe, by BioLegend (1 mentions)
Cd45ra pe cy7, by BioLegend (1 mentions)
Cd8 pb clone rpa t8, by BD (1 mentions)
2 protocols using anti human foxp3 staining set apc clone 236a e7
1
Comprehensive CD4+ T-Cell Phenotypic Analysis
2
Comprehensive Multiparametric Immunophenotyping
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The following antibodies were used: CD54 Alexa Fluor 488 (AF488), HLA class I (HLA‐I) allophycocyanin‐cyanine 7 (APC‐Cy7), CD274 phycoerythrin‐cyanine 7 (PE‐Cy7) (Biolegend), CD106 APC and HLA class II HLA‐DR APC (BD Bioscience). In cocultures, for phenotypic analysis of CD4 + ‐T, the following antibodies were used: CD4 PE (clone RPA‐T4), IFN‐γ Fluoresceine isothiocyanate (FITC) (clone B27), HLA‐DR APC (clone G46‐6), CD3 PerCP (clone SK7), CD4 Pacific Blue (PB) (Clone RPA‐T4), CD45RA PE‐Cy7 (clone H100), CD25 PE (clone M‐A251), CD127 peridinin‐chlorophyll‐protein cyanine 5.5 (PerCP‐Cy5.5) (clone A019D5), CD54 PB (clone HCD54) (Biolegend), CD8 PB (Clone RPA‐T8) (BD Biosciences), Interleukin 17 efluor660 (eBioscience). Intracellular staining of FoxP3 was carried out with the anti‐Human Foxp3 Staining Set APC (clone 236 A/E7) (eBioscience). For one experiment, each condition was tested and analyzed in duplicate or triplicate. Flow cytometry was carried out on a FACS Canto II (BD Biosciences).
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