siRNA targeting PKM2 (si-PKM2) and negative control siRNAs (si-CTL) were purchased from GenePharma (Shanghai, China). siRNA targeting COX-2 (si-COX-2) and control siRNAs were purchased from RIBOBIO (Canton, China). Plasmids were a gift by Dr. X He (19 (link)). siRNAs and plasmids were transfected by Lipofectamine 3000 (Invitrogen, CA, United States) or with Hilymax (Dojindo, Japan) following the manufacturer’s instructions.
Si cox 2
Manufactured by RiboBio
Sourced in China
Si-COX-2 is a laboratory equipment product designed for research purposes. It is a tool used to measure and analyze the expression of the COX-2 gene, which plays a role in various biological processes. The core function of Si-COX-2 is to facilitate the study and understanding of COX-2 gene expression patterns and dynamics in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Control sirna, by RiboBio (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Si pkm2, by GenePharma (1 mentions)
Si ctl, by GenePharma (1 mentions)
Hilymax, by Dojindo Laboratories (1 mentions)
Si nc, by Thermo Fisher Scientific (1 mentions)
Si nc, by RiboBio (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using si cox 2
1
Silencing PKM2 and COX-2 in Cancer Cells
2
Regulation of COX-2 expression by siRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The specific silencer RNAs (si-COX-2) and their negative controls (Si-NC) were purchased from Ribobio (Guangzhou, China). The human COX-2 gene was amplified from human genomic DNA. The COX-2 sequence was cloned into the pcDNA3.1 vector and verified by sequencing. The SGC7901 cells were seeded into plates and transfected with pcDNA3.1 (vector control), pcDNA3.1/COX-2, Si-NC, or si-COX-2 using Lipofectamine ® 2000 reagent (Invitrogen) according to the manufacturer's instructions. The final concentration in the transfection mixture was 50 nM. Three independent experiments were carried out.
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