Nxa931v

Manufactured by Cytiva

The NXA931V is a laboratory instrument designed for high-performance liquid chromatography (HPLC) applications. It is a compact and reliable device that provides precise control and monitoring of the mobile phase flow rate, pressure, and other critical parameters required for HPLC separation and analysis. The NXA931V is a core component of many analytical workflows in various scientific and industrial settings.

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Lab products found in correlation

Na934v, by Cytiva (3 mentions) Np0343, by Thermo Fisher Scientific (2 mentions) Np0321, by Cytiva (2 mentions) Wg1402, by Cytiva (2 mentions) G box imager, by Syngene (2 mentions) F3165 1mg, by Merck Group (2 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg, by Cytiva (1 mentions) Lds sample buffer, by Thermo Fisher Scientific (1 mentions) Sab2100871, by Merck Group (1 mentions)

4 protocols using nxa931v

Western Blot Protocol for Protein Analysis

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Western blots were performed as described previously^{59 (link)}. In brief, cells were lysed in NuPage LDS sample buffer (Invitrogen, NP0007) at 1 × 10⁴ cells per µl. Proteins were resolved using NuPage Bis-Tris gel electrophoresis (Invitrogen, NP0343, NP0321 or WG1402) and transferred to nitrocellulose membranes (Amersham, 10600037). Secondary antibodies were peroxidase-conjugated anti-mouse IgG (Amersham, NXA931V) or anti-rabbit IgG (Amersham, NA934V). Peroxidase activity was detected using an ECL kit (Prometheus, 20-302B) and the Syngene G-Box imager. Primary antibodies are listed below.

Nassour J., Aguiar L.G., Correia A., Schmidt T.T., Mainz L., Przetocka S., Haggblom C., Tadepalle N., Williams A., Shokhirev M.N., Akincilar S.C., Tergaonkar V., Shadel G.S, & Karlseder J. (2023). Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis. Nature, 614(7949), 767-773.

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Western Blot Protein Analysis Protocol

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Western blots were performed as described^{32 (link)}. In brief, adherent and floating dead cells were lysed in NuPage LDS sample buffer (Invitrogen #NP0007) at 1 × 10⁴ cells per μl. Proteins were resolved using NuPage Bis-Tris gel electrophoresis (Invitrogen #NP0343, #NP0321, or #WG1402) and transferred to nitrocellulose membranes (Amersham #10600037). Secondary antibodies were peroxidase-conjugated anti-mouse IgG (Amersham #NXA931V) or anti-rabbit IgG (Amersham #NA934V). Peroxidase activity was detected using an ECL kit (Prometheus #20–302B) and Syngene G-Box imager. Primary antibodies are listed below.

Nassour J., Radford R., Correia A., Fusté J.M., Schoell B., Jauch A., Shaw R.J, & Karlseder J. (2019). Autophagic cell death restricts chromosomal instability during replicative crisis. Nature, 565(7741), 659-663.

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Western Blot Antibody Concentration Protocol

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The 1° mouse anti-FLAG antibody (Sigma Aldrich Catalogue#F3165-1MG) used to detect Age2-Neon-3×FLAG was used at a 1:2000 dilution. The 1° rabbit anti-G6PDH antibody (Sigma Aldrich Catalogue#SAB2100871) was used at a concentration of 1:30,000. The 2° sheep IgG horseradish peroxidase-linked anti-mouse antibody was used at a 1:5000 dilution (Amersham catalogue#NXA931V). The 2° donkey IgG horseradish peroxidase-linked anti-rabbit antibody was used at a 1:5000 dilution (Amersham catalogue#NA934V).

Manzer K.M, & Fromme J.C. (2023). The Arf-GAP Age2 localizes to the late-Golgi via a conserved amphipathic helix. Molecular Biology of the Cell, 34(12), ar119.

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Immunoblotting with FLAG and G6PDH Antibodies

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The 1° mouse anti-FLAG antibody (Sigma Aldrich Cat #F3165–1MG) used to detect Age2-Neon-3xFLAG was used at a 1:2000 dilution. The 1° rabbit anti-G6PDH antibody (Sigma Aldrich Cat #SAB2100871) was used at a concentration of 1:30,000. The 2° sheep IgG horseradish peroxidase-linked anti-mouse antibody was used at a 1:5000 dilution (Amersham cat #NXA931V). The 2° donkey IgG horseradish peroxidase-linked anti-rabbit antibody was used at a 1:5000 dilution (Amersham cat #NA934V).

Manzer K.M, & Fromme J.C. (2023). The Arf-GAP Age2 localizes to the late-Golgi via a conserved amphipathic helix. bioRxiv.

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